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Cry1Ja对转基因昆虫控制的实用性

John P Mathis¹, Catherine Clark¹, Amit Sethi¹

¹Corteva Agriscience, Indianapolis, IN 46268, USA.

|September 27, 2024

概括

像Cry1Ja这样的新型杀虫蛋白为对抗害虫耐药性提供了新的作用模式. 这项研究评估了Cry1JaJa.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
生物化学生物化学

背景情况:

突菌 (Bt) 蛋白质对于昆虫害虫管理和作物产量至关重要.
昆虫对当前Bt特征的耐药性需要具有不同作用模式的新型杀虫蛋白.
Cry1Ja显示出杀虫活性,并在植物中对虫虫害进行保护.

研究的目的:

为了评估Cry1Ja蛋白的作用模式 (MoA).
为了比较Cry1Ja的结合部位与关键类虫害中的现有Bt蛋白.
评估Cry1Ja对抗抗其他Bt毒素的昆虫种群的疗效.

主要方法:

使用来自Helicoverpa zea,Spodoptera frugiperda和Chrysodeixis includens的刷边膜囊泡 (BBMVs) 的竞争性结合试验.
对Cry1Ja对抗Cry1Fa耐药秋季军 (FAW) 的生物活性进行评估.
对Cry1Ja对目标害虫的植物疗效的评估.

主要成果:

与其他商业Bt蛋白相比,Cry1Ja在被测试的昆虫物种中表现出明显的结合位相互作用.
Cry1Ja对抗抗抗Cry1Fa.Fa的秋季军种群具有显著的生物活性.
在植物表达Cry1Ja提供强大的保护,防止昆虫食.

结论:

Cry1Ja具有独特的作用模式,使其成为新的昆虫特征开发的有价值的候选者.
Cry1Ja可以克服类虫害中现有的抵抗机制.
这些发现支持开发新的基于Bt的昆虫控制策略.

关键词:

细菌 thuringiensis 这种疾病.克里索代克西斯包含了包括.在Cry1J蛋白质中.这是一架直升机的飞行器.它们是花鸟 (Spodoptera frugiperda) 的种类.竞争性的绑定测试试验.在植物测试中测试.杀虫剂蛋白质生物测试生物测试

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