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Updated: Jun 19, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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目标导航CBT-Cys"拼接"与CRISPR/Cas12a放大组合用于光电化学核酸测试

Jie Zheng1, Xiaoyu Wang2, Hongqing Qin1

  • 1College of Biological Engineering, College of Chemistry and Molecular Engineering, Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.

Analytical chemistry
|September 27, 2024
PubMed
概括

开发了一种使用点击化学的新型无酶DNA链接方法,使得DNA末端能够快速连接. 这种方法增强了滚动圆放大 (RCA) 和CRISPR/Cas12a切割,用于敏感的检测.

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CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...