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铜诱导的氧化应激抑制了 Aurelia coerulea 的无性繁殖

Qing Liu¹, Xiangyu Li², Qinglong Tang³

¹College of Animal Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China.

Ecotoxicology and environmental safety

|September 27, 2024

概括

铜暴露会通过诱导氧化应激来抑制Aurelia coerulea聚的无性繁殖,包括变形和芽生长. 抗氧化剂治疗逆转了这些影响,证实了氧化应激在铜中的作用.

科学领域:

海洋生物学海洋生物学
生态毒理学生态毒理学
细胞生物学细胞生物学

背景情况:

Aurelia coerulea 的息肉对于水母的生命周期至关重要.
铜是一种常见的环境污染物,具有潜在的毒性影响.
了解铜对多无性繁殖的影响对于海洋生态系统健康至关重要.

研究的目的:

为了阐明铜 (CuSO4) 抑制 Aurelia coerulea 体中无性繁殖的机制.
调查氧化应激在铜诱导的生殖抑制中的作用.
评估抗氧化剂减轻铜的不良影响的潜力.

主要方法:

露出Aurelia coerulea的息肉不同度的CuSO4.
测量氧化应激生物标志物 (ROS,MDA,CAT,H2O2,T-AOC,SOD) 的使用.
转录组分析以确定受影响的基因表达和途径.
用RT-qPCR验证关键基因表达.
应用L-亚斯科布酸来评估抗氧化作用.

主要成果:

铜以与氧化应激相关的剂量依赖的方式抑制了聚的稳定和芽,与氧化应激相关.
转录组分析显示了DNA复制和氨基酸代谢途径的改变.
抗氧化剂L-甲酸治疗减轻了铜对转化和芽的抑制作用.
氧化应激标记被抗氧化剂治疗调节,增加了ROS,MDA,CAT,H2O2和SOD,并降低了T-AOC.

结论:

暴露于铜会诱导氧化应激,从而抑制Aurelia coerulea的无性繁殖 (变形和芽).
氧化应激是介导这些生物体中铜毒性的关键机制.
酸可以抵消铜的负面影响,突出了氧化应激路径的作用.

关键词:

奥雷利亚科鲁莱亚的息肉.芽的蔓延蔓延的蔓延对铜的暴露铜的暴露变形的转化过程氧化应激是一种氧化应激.

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