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在产前暴露于黑碳和带血中DNA甲基化

Chloe Friedman¹, Sierra Niemiec², Dana Dabelea³

¹Department of Epidemiology, Colorado School of Public Health, University of Colorado Anschutz Medical Campus, Aurora, CO, USA; Lifecourse Epidemiology of Adiposity and Diabetes (LEAD) Center, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.

International journal of hygiene and environmental health

|September 27, 2024

概括

产前暴露于黑碳 (BC) 空气污染与新生儿的DNA甲基化改变有关. 这种表观遗传变化可能部分解释BCBC.

科学领域:

环境表观遗传学环境表观遗传学
发育毒理学发展毒理学
心脏代谢健康状况

背景情况:

产前暴露于环境空气污染与儿童心脏代谢不良结果有关.
之前的研究表明,产前黑碳 (BC) 和儿童脂素水平之间存在逆相关性.
假设表观遗传修饰,如DNA甲基化,可以调解子宫内暴露的长期健康影响.

研究的目的:

为了研究产前暴露于黑碳 (BC) 和带血液中的DNA甲基化模式之间的关系.
检查DNA甲基化是否调解了产前BC暴露与儿童皮菌素水平之间的关联.

主要方法:

从科罗拉多州健康开始研究中对532对母婴进行了全表观基因组协会研究.
产前环境BC暴露估计使用时空模型在母亲的住所.
通过Illumina 450K阵列测量带血中的DNA甲基化;多重线性回归用于评估与429,246个CPG的关联.

主要成果:

在多次测试校正后,44个CpG位点与产前环境BC的平均值显著相关.
通过DMRff和ENmix-combp.p两种方法确定了24个差异甲基化区域 (DMR).
一个CpG位点 (cg01123250),注释到UNC80基因中,发现它介导了大约20%的产前BC对儿童皮细胞的影响.

结论:

产前暴露于黑碳 (BC) 与带血DNA甲基化的显著改变有关.
DNA甲基化成为潜在的部分调解者,将产前BC暴露与儿童心脏代谢不良结果联系起来.

关键词:

空气污染大气污染这是带血.通过DNA甲基化.产前暴露在产前暴露.

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