相关实验视频

Updated: Jun 11, 2025

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Published on: July 30, 2020

类酸通过激活Nrf2/HO-1信号通路来促进猪卵细胞的成熟

Yan-Qiu Wang¹, Yan-Wei Dong¹, He-Xuan Qu¹

¹Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, 130062, China.

|September 27, 2024

概括

奥莱阿诺酸 (OA) 通过提高抗氧化能力和线粒体功能来增强猪卵细胞的成熟. 这种五环三烯激活了Nrf2/HO-1通路,改善了胚胎的发育.

科学领域:

生殖生物学生殖生物学
生物化学生化学
细胞生物学细胞生物学

背景情况:

烯酸 (OA) 是一种五环三烯,以其抗氧化和抗炎性质而闻名.
卵细胞的体外成熟 (IVM) 对辅助生殖技术至关重要.
了解IVM期间改善卵细胞质量的因素对于繁殖成功至关重要.

研究的目的:

研究酸 (OA) 在IVM期间改善猪卵细胞成熟的作用和机制.
为了确定OA是否提高了卵细胞发育潜力和胚胎质量.
阐明OA发挥其作用的分子途径.

主要方法:

猪卵细胞在试验室中以或没有5μM的油脂酸成熟.
评估了卵细胞成熟率,胚胎发育和胚胎细胞数量.
测量了线粒体膜潜力,ATP水平,氧化应激标志物 (ROS,GSH) 和抗氧化酶活性 (SOD,CAT).
分析了与亡相关的蛋白质 (BAX/BCL2,Cleaved Caspase3) 和Nrf2/HO-1通路组件的表达.
评估了与脂肪酸β-氧化相关的脂肪酸代谢和基因表达.

主要成果:

用5μM OA补充剂显著增加了卵细胞成熟率,伴生代激活率和体细胞核转移胚胎发育率.
甲状腺治疗导致积细胞扩张的改善和更高的胚胎囊细胞数量.
甲酸促进脂肪酸的积累,增强线粒体功能 (增加膜潜力和ATP),并降低了亡.
通过增加Nrf2,HO-1,GSH,SOD和CAT,OA显著提高了抗氧化防御的调节,同时降低了ROS水平.
氧化激活了Nrf2/HO-1信号通路.

结论:

烯酸有效地改善了猪卵细胞在体外成熟过程中的成熟和发育能力.
通过促进脂肪酸代谢,改善线粒体功能,并通过Nrf2/HO-1通路减少氧化应激,OA可以提高卵细胞质量.
甲酸是改善猪卵细胞IVM结果的有希望的补充剂.

关键词:

脂质代谢脂质的代谢 Nrf2 / HO-1 的一个.油酸是什么? 油酸是什么?氧化应激是一种氧化应激.猪卵细胞猪卵细胞

更多相关视频

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

相关概念视频

Oogenesis

Oogenesis

Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...

Hormonal Control of the Ovarian Cycle

Hormonal Control of the Ovarian Cycle

The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...

Peroxisomes

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...