毛囊刺激激素的外功能:证据表明它在内皮生理学和功能障碍中的作用
Maria Santa Rocca1, Micaela Pannella2, Erva Bayraktar3
1University Hospital of Padua, Unit of Andrology and Reproductive Medicine, Padua, Italy.
Molecular and cellular endocrinology
|September 27, 2024
概括
高度的卵泡刺激激素 (FSH) 影响内皮细胞功能,增加透性,并可能影响内皮屏障. 这表明FSH在生殖之外的作用,可能与心血管风险有关.
科学领域:
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
背景情况:
- 毛囊刺激激素 (FSH) 对于像毛囊生成和精子生成这样的生殖功能至关重要.
- FSH受体 (FSHR) 存在于外腺组织中,这表明其作用超出了生育能力.
- 以前关于FSH对内皮细胞的影响的研究产生了相互矛盾的结果.
研究的目的:
- 研究FSH在内皮细胞功能中的作用.
- 检查FSH对人静脉内皮细胞 (HUVEC) 的影响.
主要方法:
- 用复合人类FSH (rhFSH) 刺激HUVECs.
- 测量内皮氧化合成酶 (eNOS) 表达,酸化和氧化 (NO) 生产.
- 评估释放,IP3产生,NFAT1局部化和伤口愈合的测试.
- 细胞透率和VE-cadherin分布的分析.
主要成果:
- rhFSH刺激增加了HUVEC中的eNOS表达,eNOS酸化和NO生产.
- 在rhFSH刺激后,观察到释放和IP3产量的增加.
- 伤口愈合试验显示,在各种rhFSH度和时间点的细胞迁移中存在显著差异.
- rhFSH提高了细胞透性,改变了VE-cadherin分布和行为丝.
结论:
- 高度的FSH可以通过影响VE-cadherin来破坏内皮膜.
- 变化的VE-cadherin和增加的膜透性表明内皮屏障受损.
- 由于FSH的过度NO生产可能会影响内皮功能,并在特定条件下可能导致心血管风险.
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