相关实验视频

Updated: Jun 11, 2025

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

微RNA-615-3p降低了人类肝细胞中的ApoB表达

Abulaish Ansari¹, Pradeep Kumar Yadav², Swati Valmiki³

¹Department of Foundations of Medicine, New York University Grossman Long Island School of Medicine, Mineola, NY, USA; Department of Cell and Developmental Biology, Weill Cornell Medicine, New York, NY, USA; Department of Research, Weill Cornell Medicine-Qatar, Qatar Foundation, Education City, Doha, Qatar.

Journal of lipid research

|September 27, 2024

概括

研究人员发现了一种新的微RNA (miR-615-3p),可以降低阿波利波蛋白B (apoB) 水平,这是代谢疾病的关键因素. 这一发现为治疗心血管疾病提供了潜在的新策略,通过向apoB生产.

科学领域:

生物化学生物化学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

血脂质由含有阿波利波蛋白B (apoB) 的脂蛋白进行运输.
升高的apoB水平与代谢疾病和动脉样硬化心血管疾病有关.
微RNAs (miRs) 是基因表达的调节者,具有治疗潜力.

研究的目的:

为了识别调节阿波利波蛋白B (apoB) 表达的新型微RNA.
研究miR-615-3p在apoB调节和脂蛋白代谢中的作用.
探索针对代谢和心血管疾病的miR-615-3p的治疗潜力.

主要方法:

使用生物信息学和基于细胞的测试来识别miR-615-3p.
路西法酶记者测定证实了miR-615-3p和apoB mRNA 3'-UTR.之间的相互作用.
通过CRISPR-sgRNA技术调节内源性miR-615-3p水平.
在人类肝瘤 Huh-7 细胞中对 miR-615-3p 的过度表达研究.
测量细胞和分泌的apoB100,甘油三和内质网膜压力标志物.

主要成果:

miR-615-3p直接针对apoB mRNA的3'-UTR,诱导降解并降低apoB100水平.
使用CRISPR-sgRNA降低内源性miR-615-3p的调节增加了apoB100的表达.
过度表达miR-615-3p降低了细胞和分泌的apoB,并且在没有ER压力的情况下增加了细胞甘油三.
miR-615-3p作为人类肝细胞中apoB表达的负调节剂.

结论:

miR-615-3p是人类肝脏衍生细胞中阿波利波蛋白B (apoB) 表达的新型调节剂.
调节miR-615-3p水平为管理apoB相关的代谢和心血管疾病提供了潜在的治疗策略.
进一步发现调节apoB的miRs可能会揭示脂蛋白组合和分泌的新机制.

关键词:

在 apoBB 的时候.它们是阿波利波蛋白 (Apolipoproteins).心血管疾病心血管疾病脂质脂质脂质是一种脂蛋白质是一种脂蛋白质.微RNAs 是一个微型RNA.

更多相关视频

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...