一个在体内基因组编辑的小说,用于Zea mays L L的双倍的单 haploid 系统
Huaxun Ye1,2, Mei Louden1, Jon A T Reinders3
1Corteva Agriscience. Johnston Global Business Center, Johnston, IA, USA.
Nature plants
|September 27, 2024
概括
这项研究引入了一种新的体内方法,用于创建基因编辑的双倍平化 (DH) 玉米. 这种方法绕过了组织培养和化学剂,加速了玉米育种中的遗传收益.
科学领域:
- 植物遗传学和育种.
- 基因编辑技术 基因编辑技术
- 玉米 (Zea mays) 的生物学
背景情况:
- 双倍哈普洛伊德 (DH) 技术对于加速玉米近亲血统发展至关重要.
- 目前在玉米DH种群中进行的CRISPR-Cas基因编辑显示编辑频率较低.
- 高效的染色体倍增仍然是玉米DH生产中的一个重大瓶.
研究的目的:
- 开发一种改进的方法来产生基因编辑的玉米双倍平体 (DH) 种群.
- 为了克服基因编辑效率和玉米染色体倍增的局限性.
- 为了实现可扩展和具有成本效益的加速,在玉米的遗传收益.
主要方法:
- 在未受精卵细胞中,Zea mays BABY BOOM和cyclin D类基因在子宫外共同表达.
- 在体内诱导母体衍生双胞胎胚胎.
- 将基因编辑技术与体内二倍化方法的整合.
主要成果:
- 在体内成功产生母体衍生的双胞胎胚胎.
- 在没有体外组织培养的情况下生成基因编辑的玉米DH种群.
- 消除了对化学染色体倍增剂的需求.
结论:
- 已经建立了一种新的体内方法,用于创建基因编辑的玉米DH种群.
- 这种方法增强了玉米中的基因编辑和染色体倍增结果.
- 该方法预计将以可扩展和具有成本效益的方式加速玉米的遗传收益.
相关概念视频
01:20Monohybrid Crosses
Overview
01:18Dihybrid Crosses
Overview
02:38Asexual Reproduction
Asexual reproduction allows plants to reproduce without growing flowers, attracting pollinators, or dispersing seeds. Offspring are genetically identical to the parent and produced without the fusion of male and female gametes.
01:59Plant Breeding and Biotechnology
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
02:08Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
02:27Trihybrid Crosses
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...