Cdc42 移动性和膜流调节分裂酵母细胞形状和存活率
David M Rutkowski1, Vincent Vincenzetti2, Dimitrios Vavylonis3
1Department of Physics, Lehigh University, Bethlehem, PA, USA.
Nature communications
|September 27, 2024
概括
由Cdc42 GTPase活性驱动的膜流对于细胞极化至关重要. 在酵母中修改Cdc42的移动性证实了模型对流动诱导的蛋白质耗尽和极化动态的预测.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 系统生物学 系统生物学
背景情况:
- Cdc42 GTPase 是细胞极性的一个关键调节器.
- 膜相关蛋白质在细胞过程中起着至关重要的作用.
- 外细胞体和内细胞体有助于膜动力学.
研究的目的:
- 为了研究膜流在Cdc42驱动的细胞极化中的作用.
- 模拟Cdc42活动,膜流和蛋白质流动性之间的相互作用.
- 用酵母中修改的Cdc42基因组来实验验证模型预测.
主要方法:
- 开发了一种反应-扩散粒子模型,用于Cdc42激活,水解和流动诱导的位移.
- 通过修改其前化部位来改变Cdc42移动性的工程Schizosaccharomyces pombe菌株.
- 在工程酵母菌株和删除突变体中评估细胞活力和两极分化.
主要成果:
- 模型预测,低流动性GTPase激活蛋白 (GAPs) 的流动诱导的耗尽促进了两极分化.
- 实验创建的Cdc42等位基因具有流动性降低和流量合增加.
- 观察到,减少Cdc42的移动性损害了极化和活力,与模型预测一致.
- 删除Cdc42 GAP恢复了生命力,但在低移动性的Cdc42突变体中损害了两极分化.
结论:
- 膜流是Cdc42驱动的模式形成和细胞极化不可或缺的一部分.
- Cdc42-GTP的周转率必须超过有效的图案形成的表面速度.
- 蛋白质移动性,膜流和GTPase活动之间的相互作用决定了细胞极性.
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