相关实验视频

Updated: Jun 11, 2025

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

mTOR/miR-142-3p/PRAS40信号级联对于结核性硬化症复杂关联的囊形成至关重要

Shuyun Zhao¹, Shuai Hao¹, Jiasheng Zhou¹

¹Department of Pathophysiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, Liaoning, People's Republic of China.

Cellular & molecular biology letters

|September 28, 2024

概括

结核性硬化综合体 (TSC) 通过mTOR过活化引起脏问题. 我们的研究表明,抑制PRAS40或增强miR-142-3p可以减少TSC相关的囊生长.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
在瘤学瘤学.

背景情况:

结核性硬化综合体 (TSC) 的特征是TSC1或TSC2的突变,导致mTOR过活化.
这种过度活化导致脏囊和血管肌脂瘤 (AML) 的发展.
连接mTOR过活化与TSC病理的精确分子机制尚不清楚.

研究的目的:

研究PRAS40在TSC相关囊发生中的作用.
阐明涉及TSC中mTOR,miR-142-3p和PRAS40的分子途径.
探索针对这种途径的潜在治疗策略.

主要方法:

他们使用了一种小鼠模型,其中Tsc2在管中被特别淘汰 (Tsc2^f/f; ksp-Cre).
在这些小鼠中,PRAS40在全球范围内被删除以评估其功能.
使用Tsc2缺乏细胞和DNA甲基化抑制来研究miR-142-3p和PRAS40调节.

主要成果:

一个mTORC1成分PRAS40在Tc2缺乏细胞和脏中过度表达,随着mTOR抑制而下降.
通过抑制miR-142-3p,mTOR激活增加了PRAS40;升高的PRAS40推动了细胞增殖和细胞生成.
抑制DNA甲基化增加了miR-142-3p,减少了PRAS40,并抑制了囊的形成.

结论:

在TSC2缺陷状态中的mTOR激活通过miR-142-3p抑制对PRAS40进行上调.
减少PRAS40或药理上诱导miR-142-3p改善了TSC2缺陷驱动的囊形成.
针对mTOR/miR-142-3p/PRAS40轴为mTOR相关疾病提供了潜在的治疗途径.

关键词:

在PRAS40中,我们可以使用PRAS40的方法.脏细胞生成 (renal cystogenesis) 是发生在脏中的一个过程.结核性硬化综合体结核性硬化综合体在mTOROR中使用mTOR.这是一个小RNARNA.

更多相关视频

Generation of Human Kidney Tubuloids from Tissue and Urine

Generation of Human Kidney Tubuloids from Tissue and Urine

Published on: April 16, 2021

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Published on: January 31, 2025

相关概念视频

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Microtubules in Signaling

Microtubules in Signaling

The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...