在Platycodon grandiflorum的三类沙素合成途径中的关键功能基因的基于转录组的分析
Guoyu Wang1,2, Xiaoting Wan1,2, Xiaolu Li1
1College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China.
BMC genomic data
|September 28, 2024
概括
对Tongcheng Platycodon grandiflorum根的转录组测序揭示了关键的氨酸合成基因. 基因表达模式在一年生,两年生和三年生植物之间有所不同,大多数基因在一年生P. grandiflorum中高度表达.
科学领域:
- 植物基因组学 植物基因组学
- 药用植物的研究研究.
- 生物技术是生物技术.
背景情况:
- 普拉提科登大花 (P. grandiflorum) 是中国的一个重要药用草本.
- 之前的转录组研究已经存在,但对不同年龄段的Tongcheng P. grandiflorum桑素合成途径的数据是有限的.
研究的目的:
- 在不同年龄 (年,两年,三年) 的Tongcheng P. grandiflorum根上进行转录组测序和生物信息学分析.
- 识别萨波宁合成途径中的关键基因和表达模式.
- 为了解P. grandiflorum生长和氨酸生产提供基础数据.
主要方法:
- 转录组测序和数据过.
- 生物信息学分析包括通过基因本体学 (GO) 和基因和基因组 (KEGG) 丰富的京都百科全书进行差异基因表达 (DEG) 分析.
- 定量实时PCR (qRT-PCR) 用于基因表达的验证.
主要成果:
- 从742,880,616次阅读中生成了111.44Gb的清洁序列数据.
- 在一年生,两年生和三年生P. grandiflorum中鉴定出5156种差异表达的单基因.
- 在特定的对对比中注释了3509,1819和1393个DEG.
- 确定了16个参与烯生物合成途径的基因,包括HMGR和DXS等关键酶.
- QRT-PCR证实,在每年的P. grandiflorum中,大多数已识别的基因表达最高.
结论:
- 这项研究为Tongcheng P. grandiflorum在不同生长年中的根提供了新的转录基因数据.
- 这些发现为P. grandiflorum发育和胺生物合成提供了重要的生物信息洞察力.
- 这项研究为未来研究关于沙宁相关酶及其在不同生长阶段的调节奠定了基础.
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