核因子红色素2-相关因子-2的激活由Mangifera indica叶片中的氧利激活
Atif Ali Khan Khalil1, Min-Seok Woo1, Dong-Min Kang2
1Department of Pharmacology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 52727, Republic of Korea.
Antioxidants (Basel, Switzerland)
|September 28, 2024
概括
果叶提取物,特别是n-hexane分数 (MLHx),通过增加核NRF2水平来激活抗氧化途径. 从MLHx中分离出来的特定氧利平也在HeLa细胞中显示出强大的NRF2激活特性.
科学领域:
- 植物化学 植物化学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 曼果 (Mangifera indica L.) 叶子因其生物活性化合物而具有药用特性.
- 氧化应激与各种细胞功能障碍有关.
- 通过NRF2调节的抗氧化反应元素 (ARE) 途径对于细胞防御氧化应激至关重要.
研究的目的:
- 为了研究红杉叶提取物对HeLa细胞中氧化应激的影响.
- 为了确定负责观察到的抗氧化活性的特定化合物.
- 阐明了提取物保护作用背后的分子机制.
主要方法:
- 提取和分成Mangifera indica的叶子.
- 测试HeLa细胞中抗氧化反应元素 (ARE) -化酶活性.
- 对HO-1 mRNA表达和核NRF2水平的分析.
- 对ERK1/2酸化和NRF2稳定性的研究.
- 使用MS和NMR进行生物活性引导的氧利平的隔离和结构阐明.
主要成果:
- 该n-hexane部分 (MLHx) 显著增强了ARE-luciferase活性和HO-1 mRNA表达.
- 通过ERK1/2酸化和NRF2稳定,MLHx增加了核NRF2水平.
- 六种氧利平被分离和鉴定出来;3,5和6的化合物强烈刺激了剂量依赖的ARE-露西法酶活性.
结论:
- 红杉叶提取物,特别是MLHx,具有显著的抗氧化特性.
- 已确定的氧利平是负责NRF2激活的关键生物活性化合物.
- 这项研究提供了关于果叶抗氧化作用的分子机制的见解.
相关概念视频
01:31Transducer Mechanism: Nuclear Receptors
1.3K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.3K
01:07MAPK Signaling Cascades
5.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.3K
01:16Necrosis
4.4K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.4K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
02:26NF-κB-dependent Signaling Pathway
7.3K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.3K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K