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秋季军 (Spodoptera frugiperda) 的CRISPR/Cas9基因组编辑:进展和前景

Yussuf Mohamed Salum¹, Anyuan Yin¹, Uroosa Zaheer¹

¹State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

|September 28, 2024

概括

克里斯普尔/Cas9基因组编辑工具为秋季军 (Spodoptera frugiperda) 提供了先进的害虫控制. 这项技术使得功能基因研究和遗传害虫管理策略成为可能.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

秋季军虫 (Spodoptera frugiperda) 是一个主要的农业害虫,在全球范围内造成大量作物损失.
有效的害虫防治需要先进的技术解决方案.

研究的目的:

审查CRISPR/Cas9在Spodoptera frugiperda的功能基因研究中的应用.
突出CRISPR/Cas9在开发新型害虫控制策略方面的潜力.

主要方法:

在Spodoptera frugiperda中通过胚胎微注射应用CRISPR/Cas9系统.
跨越各种生物方面的功能基因分析.

主要成果:

克里斯普尔/卡斯9促进基因淘汰和表型工程在Spodoptera frugiperda.
应用包括研究杀虫剂耐药性,行为,发育和性别确定.
克里斯普尔/Cas9显示出基因驱动和精确引导无菌昆虫技术的潜力.

结论:

克里斯普尔/Cas9是一种多功能工具,用于研究类的功能基因.
它为遗传害虫控制提供了有前途的途径,包括诱导不育和破坏发展.
存在局限性,但秋季军虫管理的未来潜力很大.

关键词:

秋天的军队虫基因功能基因功能基因功能基因组编辑基因组编辑害虫控制害虫控制害虫控制害虫控制无菌昆虫技术无菌昆虫技术

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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...