在三维人类食道器官体中建模上皮质稳态和乱
Masataka Shimonosono1, Masaki Morimoto1, Wataru Hirose1
1Herbert Irving Comprehensive Cancer Research Center, Columbia University Irving Medical Center, New York, NY 10032, USA.
Biomolecules
|September 28, 2024
概括
一种新的优化介质HOME0增强了正常人食道器官的生长. 这一进步可以通过改善与患有疾病的有机体的比较,更好地建模食道疾病,如eosinophilic食道炎.
科学领域:
- 胃肠病学 胃肠病学
- 干细胞生物学 干细胞生物学
- 有机物技术 有机物技术
背景情况:
- 高级杜尔贝科的修改中级营养混合物F12 (ADF) 用于食道器官,但对于正常的人类食道器官来说是不理想的.
- 这种限制阻碍了正常和患病的食道器官之间的比较研究.
研究的目的:
- 优化基于ADF的介质,以改善正常人食道器官的生成.
- 建立研究人类食道上皮病理和疾病的模型.
主要方法:
- 利用了不朽化的食道角质细胞系 (EPC1,EPC2) 和食道活检来产生3D器官.
- 通过评估表皮生长因子 (EGF) 和转化生长因子-β (TGF-β) 信号,优化介质.
- 模拟的病理使用介质素-13 (IL-13) 或UAB30诱导上皮细胞变化.
主要成果:
- 过度的EGF和TGF-β信号限制了正常的有机体形成.
- 被命名为HOME0的优化介质显著改善了正常的人类食道器官形成.
- 在HOME0培养的有机体中IL-13和UAB30诱导了基底细胞增生,模仿疾病状态.
结论:
- HOME0介质有助于对人类食道上皮质平衡和疾病的建模.
- 这种优化的系统允许对人类食道器官与其他物种或器官的器官进行比较研究.
- HOME0支持研究食道上皮分化梯度和对炎症或信号干扰的反应.
相关概念视频
Gross Anatomy of the Stomach
The human stomach is a vital part of the digestive system, performing multiple functions. It is located within the peritoneum, a serous membrane that lines the abdominal cavity. The stomach plays a central role in processing food substances and interacts with other digestive organs through coordinated digestive processes. The stomach has a characteristic J-shape and is divided into four main regions. The cardia is the first section where the esophagus connects to the stomach and is the entry...
Anatomy of the Gastrointestinal System
The human digestive system is an intricate and essential network for nutrient absorption and waste elimination. It encompasses the gastrointestinal (GI) tract and several accessory organs.
Here's a detailed walkthrough of this complex system:
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