相关实验视频

Updated: Jun 11, 2025

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

Published on: December 22, 2020

与癌症相关的纤维细胞蛋白作为对抗结肠直肠癌的潜在目标

Ruchi Shah^1,2, Katherine A Johnson^1,2, Anna E L Lippert^1,2

¹Division of Hematology, Medical Oncology and Palliative Care, Department of Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53726, USA.

|September 28, 2024

概括

纤维细胞激活蛋白 (FAP) 是结直肠癌 (CRC) 治疗的有前途的治疗点. 这种与癌症相关的纤维细胞标志物在CRC组织中高度表达,但在健康组织中不存在,因此非常适合基于抗体的疗法.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
生物标志物发现发现

背景情况:

鉴定结直肠癌 (CRC) 抗体疗法的特定标记因缺乏独家性和丰富性而具有挑战性.
瘤微环境中的癌症相关纤维细胞 (CAF) 提供了潜在的治疗点,因为它们表达出独特的表面标记物,并且在健康组织中不存在.

研究的目的:

评估几种CAF相关蛋白在结直肠癌组织中的表达模式.
确定CRC中新型抗体介导抗癌剂的潜在治疗点.

主要方法:

在CRC和正常组织中评估了α-平滑肌肉动蛋白 (αSMA),纤维细胞激活蛋白 (FAP),波多普拉宁 (PDPN),矩阵金属蛋白酶-2 (MMP2),转基因 (TAGLN) 和THY1的表达.
分析了PDPN,MMP2和THY1与免疫相关特征的共同表达.

主要成果:

αSMA和THY1在癌症和正常组织中都呈现出很高的丰度,限制了它们的治疗潜力.
纤维细胞激活蛋白 (FAP) 在94.5%的CRC组织中检测到,在93.7%的邻近正常组织中没有检测到.
在CRC中PDPN,MMP2和THY1的高共同表达与与免疫相关的特征相关.

结论:

纤维细胞激活蛋白 (FAP) 是结直肠癌中抗体结合物的高度特定和丰富的标.
CAF蛋白质,特别是FAP,作为新型抗癌剂的治疗点具有显著的潜力.
同表达模式表明,在特定的CRC亚型中,可能存在抗体介导的免疫参与者.

关键词:

与癌症相关的纤维细胞.结肠直肠癌是什么意思纤维细胞激活蛋白质的激活蛋白质免疫组织化学免疫组织化学肝脏转移的发生.瘤标志物瘤标志物

更多相关视频

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Published on: May 14, 2021

Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids

Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids

Published on: October 21, 2022

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...