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与肉肌肉发育中的黑色素沉积相关的机制的动态转录组概况分析

Gaige Ji¹, Ming Zhang¹, Xiaojun Ju¹

¹Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province, Jiangsu Institute of Poultry Science, Yangzhou 225125, China.

Animals : an open access journal from MDPI

|September 28, 2024

概括

这项研究确定了关键基因,包括EDNRB2和DCT,这些基因在胚胎发育期间调节黑骨胸肌中的黑色素沉积. 这些发现对于理解和潜在地提高这些的经济价值至关重要.

科学领域:

基因组学和分子生物学
发育生物学发展生物学
动物科学动物科学

背景情况:

胸部肌肉变黑会影响黑骨的经济价值.
调节黑色素发生的基因在哺乳动物和鸟类中得到了很好的研究,但在胚胎发育过程中对肌肉颜色过多的情况知之甚少.

研究的目的:

为了分析Yugan黑骨胸肌中的黑色素沉积模式.
在胚胎发育过程中识别与肌肉多颜色相关的差异表达基因 (DEGs).
为了发现调节黑骨中的黑色素沉积的关键基因.

主要方法:

RNA测序 (RNA-seq) 用于在不同发育阶段 (第9,13,17,21天) 的黑骨和非黑骨胚胎之间比较基因表达.
权重基因共同表达网络分析 (WGCNA) 以确定与黑色素发生有关的基因模块和中心基因.
在化后的中,基因表达和肌肉色素 (L* 值) 之间的相关性分析.

主要成果:

在胚胎发育过程中,黑色素在肌肉纤维中逐渐积累.
在多个发育阶段确定了许多DEG,其中包括EDNRB2,TYRP1和DCT的13个常见基因.
WGCNA发现了一种"色模块",与肌肉色素发生正相关.
EDNRB2,GPNMB,TRPM1,TYR和DCT的基因表达水平与肌肉L*值显著相关.

结论:

EDNRB2,GPNMB,TRPM1,TYR和DCT是调节黑骨胸肌中的黑色素沉积的重要基因.
MSTRG.720是一种潜在的候选基因,它参与了Yugan黑骨胸肌中的黑色素沉积.

关键词:

这些DEGs是DEGs.在WGCNA中,WGCNA是WGCNA.这是一只黑骨的.黑色素沉积黑色素沉积肌肉肌肉肌肉肌肉肌肉

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