乌罗立A对牛精子容量和体外受精的影响
Manuela Jorge1,2, Filipa C Ferreira1,3, Carla C Marques1
1Unit of Biotechnology and Genetic Resources, National Institute of Agrarian and Veterinary Research, Quinta da Fonte Boa, 2005-048 Vale de Santarém, Portugal.
Animals : an open access journal from MDPI
|September 28, 2024
概括
抗氧化剂代谢物乌罗立A (UA) 通过增强运动性和减少氧化应激,改善了牛精子的功能. 这表明UAUA.
科学领域:
- 生殖生物学 生殖生物学
- 精子体生理学 精子体生理学
- 抗氧化剂研究的研究.
背景情况:
- 反应性氧物种 (ROS) 极大地影响精子功能,过多的ROS会损害受精.
- 乌罗立A (UA) 是来自ellagitannins的肠道代谢物,具有抗氧化和抗衰老的特性.
- 在此之前,UA对牛精细胞的影响还没有被研究过.
研究的目的:
- 调查乌罗立A (UA) 对冷解牛精子参数的影响.
- 评估UA对精子运动,活力,ROS水平,ATP生产和线粒体功能的影响.
- 评估UA对牛体IVF胚胎发育和质量的影响.
主要方法:
- 牛精子在不同的UA度 (0,1,10μM) 时被容纳.
- 测量了精子的运动性,活力,ROS,ATP,线粒体膜潜力和氧气消耗.
- 第二次实验评估了UA在容量和受精介质中的作用,评估了随后的胚胎发育.
主要成果:
- 1μM UA显著改善了精子的运动性 (p < 0.03).
- 补充UA增加了ATP水平 (p < 0.01),并倾向于增加氧气消耗率.
- 较高的UA度降低了ROS水平,表明线粒体功能增强和氧化应激减少.
结论:
- 乌罗立A增强牛精子的线粒体功能和代谢活性,减少氧化应激.
- 补充UA显示了改善胚胎发育和辅助生殖技术 (ART) 结果的潜力.
- 精子生物能量参数对于预测男性生育能力和ART成功至关重要.
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