将转录组学和蛋白组学结合起来,选与牛出生体重相关的候选基因
Xiuyuan Wang1, Ruili Liu1, Zhenpeng Chen1
1Laboratory of Animal Molecular, Shandong Black Cattle Breeding Engineering Technology Center, College of Animal Science, Qingdao Agricultural University, Qingdao 266109, China.
Animals : an open access journal from MDPI
|September 28, 2024
概括
研究牛胎盘功能揭示了与小牛出生体重相关的关键分子差异. 高出生体重的胎盘增强营养运输和脂质合成,而低出生体重的胎盘优先考虑细胞增殖和免疫力.
科学领域:
- 生殖生物学和分子遗传学.
- 在牲畜中比较基因组学和蛋白质组学.
背景情况:
- 胎盘对于牛胎儿发育至关重要,但其对小牛出生体重的分子调节知之甚少.
- 了解这些机制可以改善畜牧管理和生产力.
研究的目的:
- 阐明控制胎盘功能的分子机制及其对牛犊出生体重的影响.
- 确定与低出生体重 (LB) 和高出生体重 (HB) 犊相关的关键基因和蛋白质.
主要方法:
- 为LB和HB小牛胎盘创建了转录组和蛋白质组数据库.
- 使用生物信息学分析,包括功能丰富和蛋白质与蛋白质相互作用 (PPI).
- 关键的基因和蛋白质表达水平得到了验证.
主要成果:
- 在LB和HB群体之间发现了1494个差异表达的基因 (DEG) 和294个差异表达的蛋白质 (DEP).
- 乙型肝炎胎盘显示营养物质运输,能量代谢和脂质合成的增强 (例如,ELOVL5,ELOVL7,ACSL1).
- LB胎盘优先考虑细胞增殖和血管生成,具有更高的免疫标志物.
结论:
- 小牛的出生体重受胎盘营养供应,能量代谢和脂质合成途径的影响.
- ELOVL5,ELOVL7,ACSL1,CYP11A1和CYP17A1是调节小牛出生体重的潜在生物标志物.
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