炎症:分子表型的扩展和在年龄相关女性不孕症中的作用
Dmitry Ivanov1, Anna Drobintseva1, Valeriia Rodichkina2
1Deportment of Medical Biology, Saint-Petersburg State Pediatric Medical University, Litovskaya Ulitsa, 2, 194100 Saint Petersburg, Russia.
Biomedicines
|September 28, 2024
概括
细胞衰老会影响女性不孕. 子宫内膜细胞的基因毒性压力改变了衰老标志物,SIRT-1,SIRT-6和TERF下降,IL-6,IL-8和MMP3增加,这表明生殖功能障碍的新目标.
科学领域:
- 生殖生物学 生殖生物学
- 细胞衰老 细胞衰老
- 分子内分泌学分子内分泌学
背景情况:
- 细胞衰老是导致女性不孕症的一个重要因素.
- 老化相关的分泌表型 (SASP) 在与衰老相关的生殖功能障碍中起作用.
研究的目的:
- 为了研究SASP标记物和其他分子因子在人体子宫内膜细胞细胞衰老的体外模型中的表达.
- 确定年龄相关女性生殖系统功能障碍的潜在分子标记物和治疗点.
主要方法:
- 来自年轻的生殖年龄女性 (<35岁) 的初级人类子宫内膜细胞接受了基因毒性压力 (UVB/UVA辐射) 以诱导细胞衰老.
- IL-6,IL-8,IL-1α,MMP3,SIRT-1,SIRT-6,TERF-1和CALR的表达水平在mRNA (RT-qPCR) 和蛋白质 (免疫光,共聚焦显微镜) 两种水平上都被评估.
主要成果:
- 基因毒性压力显著降低了子宫内膜细胞中SIRT-1,SIRT-6和TERF-1的表达.
- 相反,IL-6,IL-8,IL-1α,MMP3和p16的表达在与未经治疗的对照细胞相比,在衰老细胞中显著增加.
- 这些分子变化表明与子宫内膜细胞诱导的细胞衰老相关的独特分子特征.
结论:
- 该研究确定了特定的分子因子 (SIRT-1,SIRT-6,TERF-1,IL-6,IL-8,IL-1α,MMP3,p16) 在人类子宫内膜细胞的细胞衰老过程中发生变化.
- 这些分子,超出已确定的SASP因子,可以作为老化相关的女性生殖功能障碍的新生物标志物.
- 已识别的分子代表了潜在的治疗点,用于解决与生殖系统衰老相关的不孕症.
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