ABA/LANCL1-2激素/受体系统通过ERRα控制心肌细胞中ROS的产生
Sonia Spinelli1, Lucrezia Guida2, Mario Passalacqua2
1Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Via Gerolamo Gaslini 5, 16147 Genova, Italy.
Biomedicines
|September 28, 2024
概括
绝缘酸 (ABA) 激素系统通过LANCL1-2受体改善心脏细胞线粒体功能和弹性. 这种ABA/LANCL系统通过ERRα.α控制H9c2细胞中的活性氧物种 (ROS) 周转.
科学领域:
- 心脏病学 心脏病学
- 分子生物学分子生物学
- 细胞生理学 细胞生理学
背景情况:
- 绝华酸 (ABA) 激素受体LANCL1和LANCL2与心肌细胞线粒体功能有关.
- 在H9c2细胞中LANCL1/2的过度表达增强了线粒体质子梯度,呼吸和低氧/低氧化后的活力.
研究的目的:
- 研究ABA/LANCL1-2系统在H9c2细胞中调节活性氧物种 (ROS) 周转中的作用.
- 为了确定转录因子ERRα在ABA/LANCL1-2介导的ROS控制中的参与.
主要方法:
- H9c2细胞被基因修改为LANCL1/2过度表达或沉默,有或没有ERRα淘汰.
- 定量实时PCR (qRT-PCR) 和西部涂抹分析了激素生产/清除的酶.
- 光探测器测量了线粒体质子梯度和ROS水平.
主要成果:
- LANCL1/2的过度表达减少了ROS生成酶,增加了ROS清除酶,并减少了线粒体ROS.
- LANCL1/2 沉声器对 ROS 营业额产生了相反的影响.
- 通过ERRα淘汰,消除了LANCL1/2过度表达对ROS新陈代谢的有益影响.
结论:
- ABA/LANCL1-2系统通过转录因子ERRα调节H9c2心肌细胞中的ROS循环.
- 该ABA/LANCL系统代表了一个潜在的治疗目标,用于增强心肌细胞线粒体健康和氧化应激弹性.
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