使用计算技术进行现场混合 (ISH) 染色图像的审查
Zaka Ur Rehman1, Mohammad Faizal Ahmad Fauzi1, Wan Siti Halimatul Munirah Wan Ahmad1,2
1Faculty of Engineering, Multimedia University, Cyberjaya 63100, Malaysia.
Diagnostics (Basel, Switzerland)
|September 28, 2024
概括
分析现场杂交 (ISH) 图像的自动计算方法显著改善了乳腺癌诊断. 这些技术,特别是深度学习,提高了HER2基因放大评估的准确性并减少了手工劳动.
科学领域:
- 数字病理学数字病理学
- 计算机成像成像技术
- 癌症生物标志物分析分析
背景情况:
- 在20-25%的乳腺癌中,HER2放大是关键的预后标志物.
- 在现场杂交 (ISH) 成像用于评估HER2状态,但由于图像异质性和复杂的生物标志物检测,面临挑战.
- 计算技术越来越多地应用于数字病理学,以加强癌症诊断.
研究的目的:
- 对分析ISH图像的半自动和全自动计算方法进行审查,重点关注HER2基因放大.
- 为了比较传统的机器学习与深度学习方法用于ISH图像分析.
- 评估这些方法在常规病理学和临床采用的潜力.
主要方法:
- 从1997年到2023年对ISH图像的计算分析进行了全面的文献综述,重点是银增强的现场杂交 (SISH).
- 对图像处理,机器学习和深度学习技术的分析,应用于HER2基因放大检测.
- 在成本效益,可扩展性和诊断准确性方面比较自动化与手动方法.
主要成果:
- 自动化ISH分析,特别是用明亮场显微镜,为病理学提供了具有成本效益和可扩展的解决方案.
- 深度学习技术显示了与传统方法相比,在HER2状态评估中提高诊断准确性的潜力.
- 在处理数据变化和对ISH分析中深度学习的计算需求方面仍然存在挑战.
结论:
- 自动化ISH分析减少了手工工作量,并提高了HER2基因放大诊断的准确性.
- 深度学习集成对推进计算病理学有前途,但需要进一步改进.
- 未来的研究应该专注于优化复杂HER2评估的计算方法,并促进临床整合.
相关概念视频
FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Immunocytochemistry and Immunohistochemistry
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
These...


