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用OPD-Cu²⁺系统基色度方法精确和独家量化生牛奶中的体细胞

Menghui Xie^1,2, Meng Wang¹, Siyuan Liu¹

¹Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.

Foods (Basel, Switzerland)

|September 28, 2024

概括

一种新的,低成本的方法使用OPD-Cu2+系统准确量化生牛奶体细胞计数 (SCC). 这种快速,用户友好的方法为评估奶牛乳腺健康和牛奶质量提供了切实可行的替代方案.

科学领域:

乳制品科学乳制品科学
分析化学分析化学
生物技术是生物技术.

背景情况:

身体细胞计数 (SCC) 对于评估奶牛乳腺健康和生牛奶质量至关重要.
传统的SCC检测方法缓慢,昂贵,容易受到细菌干扰.
需要快速,准确和用户友好的SCC量化.

研究的目的:

开发一种具有成本效益和准确的方法,用于对生牛奶体细胞计数 (SCC) 的量化.
建立一个基于OPD-Cu2+的系统,用于快速检测SCC.
评估方法的性能和对细菌干扰的抵抗力.

主要方法:

开发一个OPD-Cu2+系统用于SCC量化.
优化Cu2+和OPD度和化时间.
验证方法的准确性,线性和对细菌干扰的耐受性.

主要成果:

OPD氧化信号显示与SCC的线性相关性.
优化的系统:25μM Cu2+,2mM OPD,化时间15分钟.
方法证明了对细菌干扰的耐药性,体细胞恢复率为88.9%.
在45分钟内对10^4-10^6细胞/毫升的线性范围进行精确的SCC量化.

结论:

开发的OPD-Cu2+方法对于SCC量化具有成本效益,简单且精确.
提供了显著的实用价值和一种新的方法来检测生牛奶SCC.
为传统的,耗时的SCC评估方法提供了可行的替代方案.

关键词:

在Cu2++中.这是OPD的OPD.颜色测量试验的测试方法生牛奶生牛奶是什么意思身体细胞数量体细胞数量

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