高产量表达的人类费里重链纳米粒子在K. 对于功能性食品发展的marxianus
Xinyi Lu1,2, Liping Liu1,2, Haibo Zhang3
1State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.
Foods (Basel, Switzerland)
|September 28, 2024
概括
一般公认为安全 (GRAS) 级酵母生产高产量重组人重链费里 (rhFTH) 纳米颗粒. 这些rhFTH纳米颗粒显示出免疫增强营养产品和纳米医学应用的前景.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 纳米技术 纳米技术
背景情况:
- 一般公认为安全 (GRAS) 级微生物细胞工厂为生产基于重组蛋白质的营养产品提供了一种可持续的方法.
- 重组人重链费里丁 (rhFTH) 是一种蛋白质,在营养和医学中具有潜在的应用.
研究的目的:
- 为了利用GRAS级的*Kluyveromyces marxianus*来生产rhFTH纳米粒子.
- 评估生产的 rhFTH 的生物活动和潜在应用.
主要方法:
- GRAS等级的 *Kluyveromyces marxianus* 是为了高水平的 rhFTH.表达而设计的.
- 发酵在5升发酵器中进行,以确定产量.
- rhFTH纳米颗粒的表征,包括尺寸和铁氧化酶活性.
- 在RAW264.7小鼠细胞和*C. elegans*模型中评估含有rhFTH的酵母细胞溶解物,以检测免疫相关效应和应激反应.
主要成果:
- 在5L发酵器中实现了11g/L的rhFTH的创纪录产量.
- rhFTH形成了具有铁氧化酶活性的12nm球形纳米.
- 在小鼠细胞中,rhFTH促进了细胞因子分泌 (TNF-α,IL-6,IL-1β).
- 在压力下,rhFTH增强了运动,喉抽,产卵和*C. elegans*的寿命.
结论:
- 使用GRAS级的*K.marxianus*可实现高产量的rhFTH纳米颗粒的生产.
- rhFTH表现出显著的免疫增强和抗压性能.
- rhFTH对基于费里的新型营养产品和纳米医学中的辅助剂具有前景.
相关概念视频
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Transcription is the synthesis of RNA molecules by RNA...
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