关于八种菌体多糖的结构特征及其对胃粘膜的保护作用的研究
Haonan Wang1,2, Ying Wang1, Yuanxi Liu1
1State Key Laboratory of Drug Regulatory Science, National Institutes for Food and Drug Control, Beijing 102629, China.
Foods (Basel, Switzerland)
|September 28, 2024
概括
分析了八种Dendrobium物种多糖的结构和胃保护. DHP通过减少氧化应激和炎症来保护胃粘膜的最佳潜力.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 来自Dendrobium物种的多糖因各种生物活性而闻名.
- 了解Dendrobium多糖的结构-活性关系对于它们的应用至关重要.
研究的目的:
- 为了分析来自八个Dendrobium物种的多糖的结构特征.
- 评估这些多糖的胃粘膜保护作用.
- 为了建立多糖体结构和生物活性之间的联系.
主要方法:
- 聚糖含量和乙化程度使用紫外线分析来确定.
- 单糖化合物的组成由高性能液态染色学 (HPLC) 分析.
- 使用高性能大小排除色谱多角度光散射反射指数探测器 (HPSEC-MALS-RID) 测量了分子重量.
- 使用乙醇诱导的GES-1细胞模型评估了胃粘膜的保护.
主要成果:
- 聚糖含量从51.89%到80.57%不等,具有不同的乙化度.
- 曼诺和葡萄糖是主要的单糖,在物种之间有不同的比例.
- 所有的Dendrobium多糖都具有分子量>1 × 10^5 Da.
- 丹德罗DHP对GES-1细胞具有最显著的保护作用 (83.13%的保护),增强抗氧化酶活性,减少氧化应激标志物.
结论:
- 菌体的多糖体表现出不同的结构特征和对胃粘膜的保护作用.
- DHP具有优越的胃粘膜保护性质,可能是由于其组成和分子特性.
- 这些发现支持使用Dendrobium多糖为消化健康的营养品,并为质量控制提供基础.
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