在Pelodiscus sinensis中,通类受体表达在Aeromonas hydrophila感染后显示出不同的反应

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Yu Tian¹, Hui Zhang¹, Lingrui Ge²

¹College of Aquaculture, Hunan Agricultural University, Changsha 410128, China.

|September 28, 2024

概括

类受体 (TLRs) 在Pelodiscus sinensis中对于先天免疫非常重要. 它们对各种病原体的表达和结合能力突出显示了它们在海疾病耐药性方面的重要作用.

科学领域:

免疫学免疫学免疫学
水生动物卫生水生动物卫生
分子生物学分子生物学

背景情况:

收费类受体 (TLRs) 是关键的模式识别受体,将水生动物的先天性和适应性免疫联系起来.
在海的种类Pelodiscus sinensis中,TLRs的特定功能仍然在很大程度上未被描述.
了解TLR对于增强水产养殖中抗病能力至关重要.

研究的目的:

为了描述三种Toll类受体 (PsTLR2,PsTLR3,PsTLR5) 在Pelodiscus sinensis.
研究这些TLRs在对细菌感染的反应中的表达模式.
为了确定这些TLRs的微生物结合能力.

主要方法:

序列分析,遗传学分析和功能域预测被用来描述PsTLR2,PsTLR3和PsTLR5.
定量PCR (qPCR) 用于分析Aeromonas hydrophila感染后各种组织中的TLR基因表达.
Prokaryotic 表达被用来生产用于微生物结合测试的重组TLR蛋白质.

主要成果:

遗传学分析证实了PsTLR2,PsTLR3和PsTLR5.5的注释和正义学.
TLRs在各个组织中表达的不同,大脑/肝脏的PsTLR5高,肝脏的PsTLR2/PsTLR3高.
感染Aeromonas hydrophila诱导了TLR表达的显著变化,其中PsTLR5在脏中显示出显著的上调.
再组合的TLR蛋白显示出对一系列细菌和真菌的结合能力,包括Aeromonas hydrophila,Edwardsiella tarda,Vibrio parahaemolyticus,Staphylococcus aureus,Streptococcus agalactiae和Candida albicans等.

结论:

具有特征的TLRs (PsTLR2,PsTLR3,PsTLR5) 在Pelodiscus sinensis的先天免疫反应中发挥着重要作用.
这些TLR在微生物挑战时表现出差异化的表达模式,这表明了动态的免疫反应.
证明TLRs与各种病原体的结合表明它们在对广泛的微生物的抗性方面发挥了关键作用.

关键词:

皮洛迪斯库斯 (Pelodiscus sinensis) 是一个古老的植物.细菌感染细菌感染免疫反应的免疫反应.结合微生物的微生物结合类似于收费的受体.

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