震动EDTA,酸盐或MgSO管对血小板计数结果的影响
Michel Soulard1, Hela Ketatni1, Claire Visseaux1
1Biogroup Hematology Laboratory, La Chocolaterie, 92300 Levallois Perret, France.
Journal of clinical medicine
|September 28, 2024
概括
由于血小板凝聚,激发会显著降低酸盐和MgSO4管中的血小板数量. EDTA管显示稳定的血小板计数,使得它们在运输过程中更适合准确的血小板计数.
科学领域:
- 血液学 血液学 血液学
- 临床病理学 临床病理学
- 预分析变量 预分析变量
背景情况:
- 酸盐或硫酸 (MgSO4) 是 EDTA 诱导的伪血小板缺血症中血小板计数的替代品.
- 对于MgSO4和酸盐管的预分析条件还没有很好地定义.
- 管动荡对这些管中的血小板计数的影响需要研究.
研究的目的:
- 为了评估温和激发对K2EDTA,酸盐和MgSO4管中的血小板计数的影响.
- 为了比较不同抗凝管在激发下血小板计数的稳定性.
- 为了确定激动对血小板聚合和计数精度的影响.
主要方法:
- 在K2EDTA,酸盐和MgSO4管中收集了70名患者的样本.
- 管道在轮子上以每分钟20转的速度轻轻地动.
- 使用Sysmex XN分析仪在不同时间点分析血小板计数.
- 从基线 (T0) 计算出百分比偏差,并与EFLM偏差目标进行比较.
主要成果:
- 刺激导致血小板数显著减少:在酸盐中-29.76%和在MgSO4管中-33.18%在180分钟内.
- 在激发后,K2EDTA管显示了血小板数的最小变化 (1.17%).
- 酸盐和MgSO4管中的血小板数量下降与血小板聚合物的形成有关.
- 与震动管相比,未震动的MgSO4管显示出最小的变化 (-1.3%) .
结论:
- 激发MgSO4,特别是酸盐管,导致血小板数量下降,原因是血小板聚合物形成.
- 在EDTA管中血小板数保持稳定,即使在激发后.
- 为了确保精确的血小板结果,酸盐和MgSO4管应垂直运输,避免任何摇.
相关概念视频
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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
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