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在山羊的Mycobacterium bovis感染模型中挑战剂量定位

Elisabeth M Liebler-Tenorio¹, Nadine Wedlich¹, Julia Figl¹

¹Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut, 07743 Jena, Germany.

International journal of molecular sciences

|September 28, 2024

概括

感染Mycobacterium bovis的山羊没有出现临床症状,但产生了免疫反应和肺病变. 这些已建立的山羊模型具有剂量依赖的结果,可以帮助测试新的结核疫苗.

科学领域:

兽医医学兽医医学兽医医学
传染性疾病传染性疾病
免疫学免疫学免疫学

背景情况:

羊是Mycobacterium bovis (M. bovis) 的天然储存库,造成经济损失.
感染M. bovis的山羊模型模仿人类结核病,提供了研究潜力.

研究的目的:

在山羊中描述Mycobacterium bovis挑战模型.
建立可复制的疫苗开发模型.

主要方法:

羊被内支气管内接种了不同剂量的M. bovis.
监测临床症状,病原体分泌和免疫反应.
病变通过CT,组织学和尸体解剖后的细菌学进行了评估.

主要成果:

受感染的山羊仍然没有症状,但显示出积极的免疫反应 (IGRA,抗体,SICCT) 和T细胞激活.
M. bovis是从便中培养的,而不是从鼻子中抽取的.
在肺和淋巴结中形成的 Paucibacillary 颗粒瘤;高剂量导致较大的病变和早期泛化.

结论:

在山羊中建立了可再生的M. bovis挑战模型.
观察到剂量依赖的病变发展.
这些模型适用于评估兽医和人类结核病疫苗.

关键词:

这种细菌是Mycobacterium bovis.剂量滴定剂量调整.胸内支气管内支气管内支气管接种一只山羊,一只山羊.感染模型的感染模型结核病是一种肺结核病.

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