双S通过转化促进抗生素耐药性基因的转移
Jiayi Zhang1, Shuyao Zhu1, Jingyi Sun1
1Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
International journal of molecular sciences
|September 28, 2024
概括
双S (BPS) 是一种常见的污染物,通过增强细菌转化,显著增加了抗生素耐药性基因 (ARG) 的环境传播. 这凸显了监测BPS污染的必要性,以制抗生素耐药性危机.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 抗生素耐药性构成了全球公共卫生威胁.
- 横向基因转移,包括转换,促进了抗生素耐药性基因 (ARG) 的传播.
- 双化合物是广泛存在的环境污染物,在ARG传播中起到很大程度上尚未探索的作用.
研究的目的:
- 为了调查双S (BPS) 是否促进了由等离子体传播的ARG的转化.
- 评估环境相关的BPS度对ARG转换频率的影响.
主要方法:
- 建立了一个E.E. 大肠杆菌的DH5α转化系统使用具有抗素抗性基因的等离子体pUC19.
- 在各种BPS度 (0.1-10μg/mL) 的定量转换频率.
- 通过光探针和转录分析分析了包括反应性氧物种 (ROS) 生产,SOS反应,膜性质和ATP水平在内的细胞机制.
主要成果:
- 环境相关的BPS度提高了ARG转化频率到E. 大肠杆菌的高达2.02倍.
- BPS增加了ROS的产生,诱导了膜损伤和流动性,并削弱了等离子体吸收的屏障.
- BPS刺激了三碳酸 (TCA) 循环,增强了ATP供应和鞭毛运动性,以增强DNA搜索.
结论:
- 双S通过促进细菌转化,促进了ARG的水平传播.
- BPS改变了细菌生理学,包括膜透性和能量代谢,以增强DNA吸收.
- 监测环境BPS残留物对于减轻抗生素耐药性的传播至关重要.
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