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相关概念视频

lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
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探索大米中的林宁生物合成基因:进化,功能和表达

Munsif Ali Shad1, Xukai Li2,3, Muhammad Junaid Rao4

  • 1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, 100 Daxue Rd., Nanning 530004, China.

International journal of molecular sciences
|September 28, 2024
PubMed
概括
此摘要是机器生成的。

这项研究在大米中确定了90个素生物合成基因,揭示了基因家族通过重复扩展. 关键基因在各个组织中表达的不同,其中一些与素单体含量有关,突出显示了它们在植物细胞壁中的作用.

关键词:
细胞壁上的细胞壁.基因表达的基因表达方式基因家族是基因家族的基因家族.素单体是素的单体.米米饭 米饭 米饭 米饭.

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科学领域:

  • 植物生物学 植物生物学
  • 生物化学 生物化学
  • 遗传学 遗传学 是一个

背景情况:

  • 灵宁是血管植物中重要的生物聚合物,提供结构支持,并有助于水运输和应激反应.
  • 了解素生物合成对于改善植物细胞壁特性和生物质利用至关重要.

研究的目的:

  • 鉴定和描述涉及大米 (Oryza sativa) 素生物合成的基因.
  • 分析这些基因在各种组织和发育阶段的表达模式.
  • 为了研究基因表达和素单体含量之间的相关性.

主要方法:

  • 遗传学分析和基因构成被用来确定大米中的90个素生物合成基因.
  • 分析了33个组织样本的微阵列数据,以评估基因表达特征.
  • 使用高性能液态染色学 (HPLC) 来量化素单体.
  • 使用定量实时聚合酶链反应 (qRT-PCR) 来验证基因表达和相关性.

主要成果:

  • 确定了90个素生物合成基因,属于10个关键家族,其扩张是由重复事件驱动的.
  • 在大米组织中观察到不同的基因表达,其中一些基因始终表达,表明广泛的角色.
  • 灵宁单体含量 (H,G,S) 随着植物年龄的增长而增加,特定的基因与这些单体具有显著的相关性 (正和负).
  • 在二次生长过程中共表达了11个基因,其中6个与微阵列数据重叠,强调了它们的重要性.

结论:

  • 这项研究提供了大米红素生物合成基因的全面目录以及对其调节的见解.
  • 基因重复在素生物合成途径的进化中起着重要作用.
  • 特定的与素相关的基因对于二次细胞壁的形成和米中的素积累至关重要.