Carotid 动脉样硬化中的循环microRNAs:复杂的相互作用和与动脉血性中风的可能关联
Marine M Tanashyan1, Alla A Shabalina2, Vladislav A Annushkin1
1Research Center of Neurology, 80, Volokolamskoe Shosse, 125367 Moscow, Russia.
International journal of molecular sciences
|September 28, 2024
概括
循环中的microRNAs (miRs) 在患有冠状动脉样硬化 (CA) 和缺血性中风病史的患者中显示出改变的表达. 这些miR可以作为CA进展和中风风险的新生物标志物.
科学领域:
- 心血管研究研究心血管研究
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 动脉样硬化,特别是动脉样硬化 (CA),是心血管疾病和缺血性中风的主要驱动因素.
- 表观遗传因素,包括microRNAs (miRs),涉及CA的发病,但它们在中风中的具体作用尚不清楚.
- 了解CA患者的循环miR概况可以提供关于中风风险和疾病进展的见解.
研究的目的:
- 为了研究循环中的微RNA表达特征和症状性动脉动脉样硬化 (CA) 与缺血性中风病史之间的关联.
- 为了确定不同表达的特定miRs在CA患者之前的中风和没有中风.
- 探索这些miRs作为CA和中风风险的生物标志物的潜力.
主要方法:
- 从81名中度至重度CA.患者收集了血样本.
- 使用定量实时PCR测量了24种特定微RNA的表达水平.
- 进行了统计分析,包括多变量逻辑回归和集群分析,以确定与中风状态的显著miR关联.
主要成果:
- 在中风幸存者中,包括miR-200c-3p,miR-106b-3p和miR-494-5p在内的几种miRs被上调.
- 相反,miR183-3p,miR-126-5p和miR-216-3p在症状患者中显示出较低的血水平.
- 多变量分析确定了miR-106b-5p,miR-183-3p,miR-216-3p和miR-494-5p作为症状性CA的显著预测因素.
结论:
- 循环的microRNA表达模式在动脉动脉样硬化患者之间有所不同,有或没有缺血性中风史.
- 特定的miRs,如miR-106b-5p,miR-183-3p,miR-216-3p和miR-494-5p,是CA进展和中风的潜在表观遗传生物标志物.
- 对这些miR的进一步研究可能会导致改善心血管和脑血管疾病的诊断和预后工具.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:30Atherosclerosis I: Introduction
2
Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
2
01:30Coronary Artery Disease I: Introduction
3
Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
3
01:27Atherosclerosis II: Clinical manifestations and prevention
2
Atherosclerosis is a progressive disorder that leads to the thickening and narrowing of arterial walls due to plaque buildup. This condition can cause various symptoms depending on the arteries affected:Coronary Artery Disease (CAD): This condition affects the coronary arteries and may lead to chest pain (angina), shortness of breath (dyspnea), heart attacks, and other heart disease symptoms.Cerebrovascular Disease: This affects blood flow to the brain, causing transient ischemic attacks (TIAs)...
2