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对Populus表皮模式因子 (EPF) 家族的比较基因组学分析揭示了它们在Populus euphratica口腔发育中的调节效应

Mingyu Jia¹, Ying Wang¹, Hongyan Jin¹

¹Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Science, Tarim University, Alar 843300, China.

International journal of molecular sciences

|September 28, 2024

概括

这项研究确定了Populus euphratica中的14个表皮造型因子 (EPF) 基因,揭示了它们在口腔发育和干旱耐受性中的作用. 过度表达PeEPF2降低了口腔密度,突出了它们在植物应激适应中的重要性.

科学领域:

植物生物学植物生物学
基因组学就是基因组学.
压力生理学压力生理学

背景情况:

干旱压力对植物生长和生存有重大影响.
通过口腔调节提高用水效率 (WUE) 和耐旱能力对于作物弹性至关重要.
皮表皮肤造型因子 (EPF) /类似EPF的 (EPFL) 蛋白质是口腔发育和植物适应的关键调节者.

研究的目的:

为了识别和表征EPF基因家族成员在Populus euphratica.
调查PeEPFs在对非生物压力的反应中的进化保护和功能作用.
探索PeEPF在提高植物耐旱能力方面的潜力.

主要方法:

在Populus euphratica中对14个PeEPF基因的全基因组识别.
对基因结构,亚细胞局部化和遗传学关系的分析.
检查cis作用元素和转录基因对聚乙烯糖醇和酸处理的反应.
通过转基因系中PeEPF2过度表达的功能分析.

主要成果:

14个PeEPF基因被确定具有保留结构和保留域与阿拉比多普西斯EPF/EPFLs.
预计PeEPF蛋白在质体或细胞外定位.
对Cis-acting元素的分析表明,PeEPFs参与激素信号通路.
转录组数据显示,在干旱模拟条件下,PeEPF表达的显著变化.
过度表达PeEPF2导致转基因Populus的口腔密度降低.

结论:

PeEPF基因家族在调节Populus euphratica的口腔发育方面发挥着重要作用.
这些基因参与了植物对非生物压力的反应,特别是干旱.
PeEPF代表了基因工程的潜在目标,以改善植物对干旱的耐受性和WUE.

关键词:

在EPF基因中,EPF基因人口欧弗拉蒂克河干旱造成的压力是干旱.遗传家族鉴定遗传家庭鉴定胃的胃,就是胃的胃.

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