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染色体在长期内影响基因表达和DNA甲基化在A549细胞的体外实验中

Franziska Fischer¹, Sandra Stößer¹, Lisa Wegmann¹

¹Department of Food Chemistry and Toxicology, Institute of Applied Biosciences (IAB), Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany.

International journal of molecular sciences

|September 28, 2024

概括

长期暴露于A549细胞中的染色体会导致显著的DNA低甲基化,并改变基因表达,与短期暴露不同. 慢性暴露显示出与环境和职业环境相关的独特毒性概况.

科学领域:

环境毒理学环境毒理学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
细胞生物学细胞生物学

背景情况:

染色体暴露可以破坏表观遗传调节,包括DNA甲基化,影响基因表达和基因组稳定性.
现有的体外研究往往使用短的潜伏期,这可能不准确地反映慢性环境或职业暴露.

研究的目的:

调查短期和长期染色体暴露对人类腺癌A549细胞的差异性影响.
为了比较细胞反应,积累,细胞周期进展,DNA甲基化和基因表达在不同染色体暴露时间下.

主要方法:

在短期 (24小时) 和长期 (15周) 期间,A549细胞被不同度的染色体处理.
通过使用高通量RT-qPCR分析了细胞内积累,细胞循环停止 (G2),全球DNA甲基化水平和基因表达.

主要成果:

积累在短期暴露时显示时间和度依赖性,在长期暴露时达到平原.
短期暴露导致G2细胞循环停止,而长期低剂量暴露则没有这种情况.
长期暴露导致显著的,剂量依赖的全球DNA低甲基化,与短期暴露没有影响形成鲜明对比.
基因表达分析显示了依赖时间的变化,特别是在炎症和DNA损伤反应基因中.

结论:

短期和长期的染色体暴露在A549细胞中引起了不同的毒性概况.
长期暴露与显著的表观遗传变化有关,如DNA低甲基化和改变的基因表达模式,突出显示了它对风险评估的更大相关性.

关键词:

对DNA损伤的反应反应通过DNA甲基化.染色体的染色体是什么这是表观遗传学.基因表达特征基因表达特征这是一种炎症炎症炎症炎症.长期的风险是长期的.氧化应激是一种氧化应激.

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