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聚乙烯微/纳米塑料暴露诱导人体支气管和气泡膜上皮细胞的上皮-介质细胞过渡

Alice Traversa¹, Emanuela Mari¹, Paola Pontecorvi²

¹Department of Life Sciences, Health and Health Professions, Link Campus University, 00165 Rome, Italy.

International journal of molecular sciences

|September 28, 2024

概括

聚乙烯微/纳米塑料 (MNPs) 不会导致细胞死亡,但会改变呼吸道细胞. 吸入这些塑料颗粒可能会促进细胞迁移,并可能导致肺癌.

科学领域:

环境健康环境健康
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

微/纳米塑料 (MNP) 是普遍存在的环境污染物.
吸入是MNP进入人体的主要途径,造成健康风险.
聚乙烯 (PE) MNPs对呼吸系统的细胞影响尚不清楚.

研究的目的:

研究聚乙烯微/纳米塑料 (PE MNPs) 对呼吸道上皮细胞的影响.
评估PE MNPs对细胞活力,形态和迁移/入侵的影响.
探索潜在的分子机制,包括上皮层-介质酶过渡 (EMT).

主要方法:

呼吸道上皮细胞暴露于不同度的PE MNPs.
使用MTT测定评估的细胞活力.
细胞迁移和入侵通过抓取和跨井测试进行评估.
通过免疫光学观察到的形态变化.
通过西欧斑块和qRT-PCR分析的分子变化.

主要成果:

对PE MNPs的急性暴露没有诱导显著的细胞毒性.
PE MNPs引起了可观察到的形态变化,包括actin细胞骨架重组.
皮质-介质细胞过渡 (EMT) 被确定为一个关键的过程.
观察到细胞迁移潜力的显著增加.
细胞的入侵能力基本保持不变.

结论:

吸入PE MNPs可能会改变呼吸道上皮细胞的行为,但不会引起立即的毒性.
观察到的细胞迁移和EMT的增加表明在呼吸道致癌症发生中可能发挥作用.
需要进一步的研究,以充分阐明PE MNP暴露的长期健康影响.

关键词:

在EMT中,EMT是EMT.许多国家和地区的MNP.气管的气管的支气管是支气管中的一个.聚乙烯聚乙烯的使用情况.

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