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针和分支特征变异分析和相关SNP位置采矿在Larix olgensis中

Ying Cui¹, Jiawei Yan², Luping Jiang¹

¹College of Forestry and Grassland, Jilin Agricultural University, Changchun 130118, China.

International journal of molecular sciences

|September 28, 2024

概括

研究人员使用全基因组关联研究 (GWAS) 在Larix olgensis中确定了161个与针和分支特征相关的显著单核酸多态 (SNP) 位点. 这些发现为基因改进这些重要的树木特征铺平了道路.

科学领域:

森林遗传学森林遗传学
植物基因组学植物基因组学
定量遗传学是一种定量遗传学.

背景情况:

针和树枝对于Larix olgensis的生长和光合作用至关重要.
这些特征的遗传基础在很大程度上仍未被探索.
了解遗传变异是提高树木表现的关键.

研究的目的:

为了识别遗传变异和挖掘单核酸多态化 (SNP) 标记,与Larix olgensis的针和分支特征相关.
为了发现特征,进行全基因组关联研究 (GWAS).
开发用于繁殖计划的分子标记物.

主要方法:

131种Larix olgensis基因型的全基因组再测序 (WGRS).
对七个针头和一个分支的特征进行表型数据收集.
全基因组关联研究 (GWAS) 使用一般线性模型 (GLM).
使用竞争性等位基特异性PCR (KASP) 标记物验证显著的SNP位点.

主要成果:

总共确定了243,090,868个SNP位点.
发现了161个与针状和枝状特征有显著关联的SNP位置.
验证了20个KASP标志物,其中3个与表型变异有显著关联.
BSBM01000635.1_4693780,BSBM01000114.1_5114757和BSBM01000114.1_5128586已经成功地得到了放大和多态化.

结论:

开发了与关键Larix olgensis特征相关的新型KASP标记.
这些标记物有可能在育种计划中用于标记物辅助的选择.
促进了Larix olgensis中针状和枝状特征的遗传改进.

关键词:

在GWAS中,GWAS就是GWAS.这就是KASP标志的标志.拉里克斯老鼠的起源国家统一计划 (SNP) 是一个国家统一计划.分支机构的特征分支机构的特征针的特征是针的特征.

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