相关实验视频

Updated: Jun 11, 2025

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Published on: February 3, 2023

评价[68Ga]Ga-DOTA-AeK作为一种潜在的成像工具,用于PET成像,用于细菌感染中的细胞壁合成

Palesa C Koatale^1,2, Mick M Welling³, Sipho Mdanda^1,2

¹Department of Nuclear Medicine, University of Pretoria, Pretoria 0001, South Africa.

Pharmaceuticals (Basel, Switzerland)

|September 28, 2024

概括

研究了使用光AeK-NBD和放射性[68Ga]Ga-DOTA-AeK的细菌酸糖循环. 对于PET/CT成像,AeK的细胞内吸收至关重要,但[68Ga]Ga-DOTA-AeK显示细菌局部局限,这表明需要替代放射标签.

科学领域:

微生物学微生物学
放射化学放射化学是指辐射化学.
医疗成像医学成像

背景情况:

细菌利用外源性来进行糖生物合成.
像AeK-NBD这样的光探针能够对细菌通路进行光学成像.
pozitron发射断层扫描/计算机断层扫描 (PET/CT) 提供了体内成像的潜力.

研究的目的:

通过PET/CT开发和评估[68Ga]Ga-DOTA-AeK用于体内细菌感染成像,使用PET/CT.
为了评估与AeK-NBD相比,[68Ga]Ga-DOTA-AeK的细菌吸收和局部化.
为了确定Ga-68适用于细菌二醇糖回收的PET/CT成像.

主要方法:

用-68 ([68Ga]) 和净化对DOTA-AeK进行放射性标记.
在体外评估[68Ga]Ga-DOTA-AeK的稳定性和蛋白质结合.
使用[68Ga]Ga-DOTA-AeK和AeK-NBD进行活体和热杀死的大肠杆菌和金黄色葡萄球菌的体外细菌吸收比较研究.
在慢性感染/炎症模型中进行初步的体内生物分布研究.

主要成果:

用[68Ga]GaCl3成功对DOTA-AeK进行放射性标记,从而产生高放射性化学纯度 (≥95%).
良好的体外稳定性和[68Ga]Ga-DOTA-AeK的低蛋白质结合.
在E. coli或S. aureus中没有确的[68Ga]Ga-DOTA-AeK在体内细胞内吸收.
AeK-NBD在活体细菌中显示出显著更高的细胞内结合.
在感染部位的局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部局部

结论:

对于成功的细菌特异性PET/CT成像,AeK的细胞内结合至关重要.
[68Ga]Ga-DOTA-AeK不适合用于跟踪细菌的AeK吸收,这是因为庞大的DOTA化剂可能阻碍了膜传输.
未来的优化应该探索替代的AeK结构,这些结构可以被放射性标记,使用寿命较短的同位素,如-18 (18F) 或碳-11 (11C).

关键词:

在PET成像中使用PET成像.在PET上使用PET追踪剂.细菌感染细菌感染细胞壁上的细胞壁.净化净化净化净化质量控制质量控制质量控制综合合成是一种合成.

更多相关视频

Investigations on the GaIII Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue

Investigations on the GaIII Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue

Published on: August 17, 2016

An Automated Radiosynthesis of [68Ga]Ga-FAPI-46 for Routine Clinical Use

An Automated Radiosynthesis of [⁶⁸Ga]Ga-FAPI-46 for Routine Clinical Use

Published on: May 24, 2024

相关概念视频

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET

Positron Emission Tomography

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...