微生物组和微生物纯培养研究揭示共生微生物缓解Salmonella enterica 的血清虫感染
Jianshen Zhu1, Jinmei Ding1, Kaixuan Yang2
1Shanghai Key Laboratory of Veterinary Biotechnology, Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Microorganisms
|September 28, 2024
概括
沙门氏菌 (Salmonella Pullorum) 感染会破坏肠道微生物群,减少多样性并丰富有害细菌. 一种Lacticaseibacillus paracasei菌株在体外和体内有效地抑制了S. Pullorum.
科学领域:
- 微生物学 微生物学
- 动物科学动物科学
- 肠道微生物组研究研究
背景情况:
- 虫病是由Salmonella enterica serovar pullorum (S. Pullorum) 引起的,对家禽产业构成重大经济威胁.
- 目前的控制方法,如根除计划和抗生素的使用是昂贵的,并面临限制.
- 调节肠道微生物群为疾病控制提供了一个有希望的替代策略.
研究的目的:
- 为了研究S. Pullorum感染对肠微生物群的组成和结构的影响.
- 为了识别潜在的有益的微生物来控制S. Pullorum感染.
- 探索通过微生物群调节来管理pullorum疾病的新策略.
主要方法:
- 使用16S rRNA测序对感染S. Pullorum的肠道微生物群动态进行分析.
- 阿尔法和β多样性分析 (例如,PCoA) 来评估微生物社区结构的变化.
- 候选益生菌菌株的隔离和体外/体内测试.
主要成果:
- 普勒勒姆菌感染显著降低了肠道微生物的丰富性和多样性 (观察到的OTU,香农指数).
- 在受感染和未受感染的群体之间观察到肠道微生物群的明显聚类.
- 在受感染的中发现了埃舍里希亚-希格拉和菌的丰富,以及乳酸菌丰度的减少.
- 乳酸酶亚菌 (L. paracasei) Lp02菌株在体外表现出S. Pullorum的抑制,并在体内减轻了感染.
结论:
- 普勒勒姆菌 (S. Pullorum) 感染深刻地改变了肠道微生物群,减少了像乳酸菌 (Lactobacillus) 等有益细菌.
- 已识别的L. paracasei菌株显示出对控制pullorum病的益生菌的潜力.
- 准肠道微生物群为管理家禽S. Pullorum感染提供了一个可行的替代策略.
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