相关实验视频

Updated: Jun 11, 2025

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Published on: August 31, 2022

使用元病毒基序测序对蚊子病毒组病毒覆盖的探索:系统性审查和元分析

Shenglin Chen¹, Yuan Fang^1,2, Ryosuke Fujita³

¹National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, China.

|September 28, 2024

概括

蚊子病毒体的超病毒基序测序覆盖范围因属而异,Culex显示病毒读数比例最高. 预处理方法显著影响蚊子中的病毒检测.

科学领域:

病毒学病毒学
昆虫学昆虫学是一门学科.
生物信息学是一种生物信息学.

背景情况:

蚊子携带各种病毒,对公众健康构成风险.
超病毒基序测序是发现关节动物中新型病毒的强大工具.
了解影响测序覆盖范围的因素对于准确的病毒组表征至关重要.

研究的目的:

为了评估蚊子病毒组覆盖范围的程度,使用元病毒组测序.
识别导致病毒读数比例变化的因素.
优化检测蚊子载体中的RNA病毒的协议.

主要方法:

对蚊子病毒组的meta-viromic测序研究进行系统审查.
从多个科学数据库 (PubMed,科学网,Embase,Scopus,科学直接,谷歌学者,CNKI) 中提取数据.
随机效应建模用于聚合覆盖率估计和对异质性的子组分析.

主要成果:

在所有蚊子属中,病毒覆盖率不同:Culex (7.09%),Anopheles (5.28%) 和Aedes (2.11%).
预处理步骤,包括盐水缓冲剂/抗生素治疗和宿主基因组去除,显著影响了病毒覆盖率.
特定的处理方法对检测RNA病毒产生了重大影响.

结论:

蚊子病毒覆盖范围取决于品种,并受到元病毒基序测序协议的影响.
优化样品预处理对于最大限度地提高蚊子病毒检测至关重要.
这项研究为增强在蚊子载体中的RNA病毒发现提供了关键的见解.

关键词:

这是一个元分析.甲基因组学分析的方法蚊子病毒体的病毒体蚊子传播的病毒.

更多相关视频

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Published on: April 12, 2017

相关概念视频

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...