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EIN/EIL家族的表征和转录组分析以及它们在内部节点成熟中的作用的识别

Min Tu¹, Yuqing Hua¹, Ti Shao²

¹Hubei Technical Engineering Research Center for Chemical Utilization and Engineering Development of Agricultural and Byproduct Resources, School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China.

Plants (Basel, Switzerland)

|September 28, 2024

概括

马双色 EIL 转录因子,被确定为八个通过全基因组复制扩展的 SbEIL,通过差异表达表现出分歧. SbEIL7与内部节点成熟和蛋白质降解有关.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
乙烯信号传输乙烯信号传输

背景情况:

乙烯不敏感3/乙烯不敏感3类蛋白 (EIN3/EIL) 是调节植物对乙烯反应的关键转录因子.
EIL家族的进化历史和在 Sorghum bicolor 中的特定作用尚未得到充分理解.

研究的目的:

为了识别和表征Sorghum bicolor中的EIL基因家族.
研究SbEIL基因的演变和分歧.
探索SbEILs在内部节点开发中的作用.

主要方法:

使用生物信息学方法识别SbEIL基因.
分析蛋白序列,域架构和图案模式.
基因表达造型使用来自内部节的RNA-seq数据.
对比表达分析和共同表达网络的构建.

主要成果:

在 Sorghum bicolor 中发现了8个SbEIL基因,通过全基因组复制 (WGD) 进行扩展.
与WGD重复的SbEILs主要通过差异表达模式表现出差异.
SbEIL3和SbEIL7在内部节点中高度表达,其中SbEIL7可能参与内部节点成熟.
SbEIL7与与乌比奎丁相关的基因表现出共同调节,这表明它在蛋白质降解中的作用.

结论:

通过WGD扩展了SbEIL基因家族,其分歧是由表达模式驱动的.
SbEIL7在内部节点的成熟中起着重要作用,可能参与蛋白质降解途径.
这些发现为未来关于乙烯信号和改进的研究提供了基础.

关键词:

EIN/EIL家族号码/EIL家庭号码通过RNA-seq分析进行分析.共同表达的分析分析.基因扩张基因扩张内部节点的成熟期.这就是. sorghum.转录因子是一种转录因子.

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