天体细胞的代谢偏好:功能代谢映射揭示了丁酸盐的结果与乙酸盐的竞争
Aishat O Ameen1, Sebastian W Nielsen1, Martin W Kjær1
1Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
概括
短链脂肪酸如酸盐可能为大脑提供燃料. 星球细胞是酸盐代谢的关键,为大脑健康提供潜在的替代能源,即使在阿尔茨海默氏病模型中也是如此.
科学领域:
- 神经科学是一个神经科学.
- 代谢途径 代谢途径
- 肠-大脑轴研究研究
背景情况:
- 肠-大脑轴的干扰与神经退行性疾病有关.
- 在阿尔茨海默病 (AD) 鼠标模型中观察到减少的短链脂肪酸 (SCFA),包括丁酸盐.
- 酸盐补充剂显示出减轻AD类病理和改善记忆力的潜力,但机制尚不清楚.
研究的目的:
- 为了研究丁酸盐对大脑能量代谢的影响.
- 为了确定参与大脑内的丁酸盐代谢的主要细胞类型.
- 在阿尔茨海默病的小鼠模型中评估丁酸盐代谢.
主要方法:
- 动态同位素标记用[U-13C]酸和[1,2-13C]酸在老鼠大脑切片,初级星球细胞和神经元培养物和体内.
- 质谱测量用于绘制代谢途径的地图.
- 在5xFADADAD模型小鼠中进行代谢竞争测试和体内研究.
主要成果:
- 天体细胞优先代谢丁酸盐而不是神经元.
- 丁酸盐在代谢方面与酸盐竞争,但与体β-基丁酸盐不竞争.
- 在5xFAD小鼠中,与乙酸盐相比,丁酸盐的代谢维持和升高,无论AD病理如何.
- 在野生类型和5xFAD大脑之间没有观察到TCA循环代谢物标记的显著差异.
结论:
- 星球细胞在大脑的酸盐代谢中起着至关重要的作用.
- 丁酸盐作为一个可行的替代性脑燃料来源.
- 持续的丁酸盐代谢发生在AD模型中,表明治疗潜力.
更多相关视频
09:42Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
Published on: August 7, 2013
10.3K
06:43A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes
Published on: February 5, 2018
12.1K
相关概念视频
01:20Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
01:28Overview of Fatty Acid Metabolism
30.2K
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
30.2K