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Patryk Strzelecki1,2, Nicolas Joly3, Pascal Hébraud1

  • 1Institut de Physique et Chimie des Matériaux de Strasbourg, CNRS UMR 7504, Université de Strasbourg, 23, rue du Loess, 67000 Strasbourg, France.

Nucleic acids research
|September 28, 2024
PubMed
概括

金门组装 (GGA) 使用IIS型酶进行高效的分子克隆. 这项研究表明,强大的DNA突起增强了GGA效率,与之前的假设相反,优化了复杂的DNA组装.

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Frames

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Frames: Problem Solving I01:24

Frames: Problem Solving I

Consider a jib crane with an external load suspended from the pulley. The dimensions of the crane members are shown in the figure. A systematic analysis of the frame structure is required to determine the reaction forces at the pin joints, assuming that the pulleys are frictionless.
Frames: Problem Solving II01:26

Frames: Problem Solving II

Consider a hydraulic hoist supporting a load of 1 kN. Assuming a simplified schematic representation of this frame structure, the force acting on BD and BF members can be determined.
Design Consideration01:22

Design Consideration

Designing a structure involves a series of considerations, primarily the material's ultimate strength, calculated through tests that measure changes under increased force until the material reaches its breaking point or limit. The ultimate load, where the material breaks, is divided by its original cross-sectional area, resulting in the ultimate normal stress or strength. The ultimate shearing stress is another significant factor taken into account.
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Design of Prismatic Beams for Bending01:23

Design of Prismatic Beams for Bending

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Posttensioned Masonry Walls01:15

Posttensioned Masonry Walls


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