净化,来源勘探,和生物活性评估的Oligosaccharides在西子中
Xiaonan Chen1, Zongjin Pu1, Yixin Liu1
1School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
Journal of separation science
|September 28, 2024
概括
鉴定出SJZD-OGS为具有抗炎和细胞保护作用的果果-寡糖. 这项研究阐明了传统中医药 (TCM) 中的活性成分.
科学领域:
- 药理学 药理学是指药理学的学科.
- 生物化学 生物化学
- 传统中国医学 (TCM) 是一种
背景情况:
- 西子 (SJZD) 是一种传统的中医药,用于治疗缺陷综合征.
- 多糖和非多糖 (NPS) 是SJZD的关键活性成分.
- 寡糖在SJZD的NPS中的特殊作用需要进一步调查.
研究的目的:
- 描述从SJZD的非多糖分 (SJZD-OGS) 中分离出来的寡糖的组成和生物活性.
- 探索SJZD-OGS的起源和潜在的治疗应用.
主要方法:
- 从SJZD-NPS中分离和净化寡糖类.
- 使用超高性能液态染色学-离子移动性光谱学-四极飞行时间质谱学 (UHPLC-IMS-QTOF/MS),MALDI-TOF-MS和HPAEC-PAD的分析.
- 对巨细胞的抗炎活性和对肠道上皮细胞的保护作用的评估.
主要成果:
- 鉴定SJZD-OGS为果糖-寡糖化合物 (FOS) 的混合物,其聚合度 (DP) 从3到14不等,总分子量为2178Da.
- SJZD-OGS的寡糖化合物成分与SJZD中的单个草药不同.
- SJZD-OGS显示出显著的抗炎性质和对肠道上皮细胞的保护作用.
结论:
- 该研究阐明了SJZD内部的特定寡糖化合物成分,主要将其确定为FOS.
- 由于其抗炎和细胞保护作用,SJZD-OGS表现出有前途的治疗潜力.
- 这项研究为了解SJZD的活性药物成分提供了基础,并为研究TCM中的寡糖类提供了战略.
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