相关实验视频
Updated: Jun 11, 2025

09:56
Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
Published on: September 6, 2019
6.7K
一子酶级联用于毒剂降解和糖酸生产
Vinutsada Pongsupasa1, Pangrum Punthong1, Pimchai Chaiyen1
1School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology, (VISTEC), 555 Wangchan Valley, Rayong, 21210, Thailand.
Chembiochem : a European journal of chemical biology
|September 28, 2024
概括
这项研究介绍了一种单一的酶级联,将农药转化为电子受体,然后产生宝贵的糖酸,如乳酸和酸. 这种可持续的方法为工业提供了更高的产量和有价值的产品.
科学领域:
- 生物技术是生物技术.
- 酶催化酶的催化作用
- 绿色化学 绿色化学
背景情况:
- 农药毒性和环境污染带来了重大挑战.
- 需要可持续的方法来将危险物质转化为有价值的化学物质.
- 酶级联为高效生物转化提供了一个有前途的途径.
研究的目的:
- 开发一种新型的一子酶级联,用于将有毒物质转化为电子受体.
- 为了利用产生的电子接受器生产糖酸.
- 证明这种方法的技术可行性和好处.
主要方法:
- 一种使用有机酸盐化酶 (OPD) 和HadA单氧化酶 (HadA) 的酶级联来将农药转化为1,4-二.
- 使用1,4-基作为电子受体与pyranose脱酶 (PDH) 进行糖酸合成.
- 乳糖转化为乳酸和酸转化为2-基托-酸 (进一步减少为酸).
主要成果:
- 布成功地从乳糖中产生了乳酸和从树脂酸中产生了氧酸.
- 与直接添加1,4-基相比,单反应实现了高达10%的更高产量.
- 合成的西隆酸表现出优异的吸水特性;乳酸显示出抗氧化活性.
结论:
- 开发的酶级联是一种有效和高效的方法,用于将有毒物质提升为有价值的糖酸.
- 这种方法为农药修复和化学制造提供了一个可持续的替代方案.
- 生产的糖酸具有适用于水凝和抗氧化剂的特性.
相关概念视频
Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
Bioremediation
18.2K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.2K
Glycolysis: Pay-off Phase
9.8K
So far, glycolysis has cost the cell two ATP molecules and produced two small, three-carbon sugar molecules. These molecules will proceed through the second half of the pathway, and sufficient energy will be extracted to pay back the two ATP molecules used as an initial investment and produce a profit for the cell of two additional ATP molecules and two even higher-energy NADH molecules.
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
9.8K
Catalytically Perfect Enzymes
3.9K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Most enzymes...
Most enzymes...
3.9K
Dehydration of Aldols to Enones: Acid-Catalyzed Aldol Condensation
2.2K
As shown in Figure 1, under acidic conditions, the β-hydroxy ketone undergoes dehydration via an E1 elimination reaction to form an enone.
2.2K

