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烯增强了大米植物对刺穿吸的草食动物和大米病原体的抵抗力

Chang-Lai Qiu¹, Wei Li², Ling-Nan Wang²

¹College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

Plant biotechnology journal

|September 28, 2024

概括

米合成酶 (OsTPS19和OsTPS20) 调节 (S) - 烯排放,增强植物对棕色植物 (BPH) 和病原体的防御能力. 过度表达增强了耐药性,而淘汰则减少了耐药性,影响了害虫和疾病的发生率,但没有影响到水产量.

科学领域:

植物生物化学植物生物化学
分子生物学分子生物学
农业科学农业科学

背景情况:

合成酶 (TPS) 是 terpenoid 合成中的关键植物酶,对于抵御生物压力至关重要.
植物衍生的挥发性有机化合物,包括类,介导与害虫和病原体的相互作用.

研究的目的:

在米 (Oryza sativa) 中功能性表征OSTPS19和OSTPS20.
研究OsTPS19和OsTPS20在植物防御机制中对棕色植物 (BPH) 和主要的病原体的作用.

主要方法:

在BPH攻击下对OsTPS19和OsTPS20的基因表达分析.
产生和分析过度表达或缺乏OSTPS19和OSTPS20的转基因大米谱系.
对 (S) - 烯的BPH行为反应的评估.
对Rhizoctonia solani和Xanthomonas oryzae的抗病能力的评估,以及对害虫和疾病发病率的实地试验.

主要成果:

在BPH感染后,OsTPS19和OsTPS20的表达增加.
过度表达OsTPS19/OsTPS20的水植物释放出更多的 (S) - 烯,显示BPH卵化减少,膜炎和细菌炎的病变长度降低,BPH,爆发和膜炎的现场发病率降低.
淘汰赛线表现出对病原体的敏感性增加.
(S) - 烯度影响了BPH行为,在高度时避免使用.
过度表达并没有显著影响米产量特征.

结论:

OsTPS19和OsTPS20是大米中 (S) - 烯排放的关键调节剂.
这些酶在增强大米对BPH和R. solani和X. oryzae等病原体的防御方面发挥着重要作用.
OsTPS19和OsTPS20代表了在米育种计划中改善作物耐药性的潜在目标.

关键词:

这是一种Oryza sativa.棕色的植物跳草食动物诱导的植物挥发物.氨酸 (Limonene) 是一种氨酸烯合成酶是一种烯合成酶.植物病原体耐药性的耐药性

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