Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mitochondrial Membranes01:45

Mitochondrial Membranes

9.1K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
9.1K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

12.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
12.1K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

7.2K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.2K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

2.5K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.5K
Mitochondria01:37

Mitochondria

11.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
11.4K
Peroxisomes01:24

Peroxisomes

11.2K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
11.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Ontogenetic variation in Milos viper (Macrovipera schweizeri) venom composition and biochemical activity.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
Same author

Mitochondrial super complexes in the colorless chlorophycean alga Polytomella parva.

Journal of bioenergetics and biomembranes·2026
Same author

Monoallelic PSMB8 variants cause PRAAS with immunodeficiency through impaired immunoproteasome assembly.

American journal of human genetics·2026
Same author

The conjugation-resistant bile acid norUDCA cures liver fibrosis but impairs systemic energy metabolism.

Molecular metabolism·2026
Same author

JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.

EMBO molecular medicine·2026
Same author

Enhancing KLF15 activity in cardiomyocytes: a novel approach to prevent pathological reprogramming and fibrosis via nuclease-deficient dCas9VPR.

Signal transduction and targeted therapy·2026
Same journal

Corrigendum to: "NO modulates human airway smooth muscle function by altering glucose-6-phosphate dehydrogenase effects on sGC function in asthma" [Redox Biology 95 (2026) 104262].

Redox biology·2026
Same journal

Inhibiting 15-PGDH restores redox homeostasis and confers neuroprotection in Parkinson's disease.

Redox biology·2026
Same journal

Insights into taurine therapy for periodontitis: Targeting osteocyte ferroptosis to mitigate obesity-exacerbated bone damage.

Redox biology·2026
Same journal

Glutathione metabolism-linked ferroptosis in human seminoma: a spatial multi-omics mapping study.

Redox biology·2026
Same journal

Apurinic/apyrimidinic endonuclease 1 prevents oxidative DNA damage in intestinal epithelial cells induced by genotoxic Escherichia coli NC101.

Redox biology·2026
Same journal

Low serum selenium combined with SELENOP-autoantibodies are associated with persistent fatigue after SARS-CoV-2 infection.

Redox biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 11, 2025

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
07:17

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

Published on: July 30, 2019

7.8K

菲西奥西亚重新连接了线粒体复合物的组成,以保护干细胞的活力.

Janice Raabe1, Ilka Wittig2, Patrick Laurette3

  • 1Institute of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.

Redox biology
|September 28, 2024
PubMed
概括
此摘要是机器生成的。

人类诱导多能干细胞 (hiPSCs) 的体氧培养 (5% O2) 增强了染色体稳定性和多能性. 这种方法减少了氧化酸化和衰老,优化了研究的hiPSC功能.

关键词:
复杂的综合体 复杂的综合体这就是HIF1α.人类诱导的多能干细胞干细胞.线粒体功能 线粒体功能在NDUFA4L2中.衰老是一种衰老.

更多相关视频

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
10:05

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

1.9K
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

14.2K

相关实验视频

Last Updated: Jun 11, 2025

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
07:17

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

Published on: July 30, 2019

7.8K
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
10:05

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

1.9K
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

14.2K

科学领域:

  • 干细胞生物学 干细胞生物学
  • 细胞呼吸 细胞呼吸
  • 基因组学和蛋白质组学

背景情况:

  • 人类诱导多能干细胞 (hiPSCs) 对于研究人类生物学至关重要.
  • 在hiPSC培养过程中氧气水平显著影响细胞活力和功能.
  • 了解最佳的氧气条件对于保持hiPSC质量至关重要.

研究的目的:

  • 调查物理氧化 (5% O2) 与高氧化 (20% O2) 培养对hiPSCs的机制影响.
  • 评估对染色体稳定性,多能性和细胞代谢的影响.
  • 阐明线粒体功能和蛋白质组成中的分子变化.

主要方法:

  • 从52个个体的皮肤纤维细胞重新编程成hiPSC.
  • 在物理氧 (5% O2) 和高氧 (20% O2) 条件下的培养.
  • 蛋白质基因分析,Giemsa-banding用于型定型,特定阶段胚胎抗原3 (SSEA-3) 染色,葡萄糖/乳酸盐测定,RNA-seq,ATAC-seq和线粒体测定.

主要成果:

  • 身体性高PSCs显示显着较少的染色体马赛克 (6%对32%) 和更高的SSEA-3阳性.
  • 物理氧培养导致氧化酸化降低,衰老标志物降低 (IGFBP3,β-galactosidase),并改变了线粒体复合体组成 (例如,NDUFA4L2,ATP5IF1).
  • RNA-和ATAC-seq揭示了低氧转录因子结合足迹,包括增加的NDUFA4L2表达和染色质可访问性.

结论:

  • 与高氧条件相比,体氧培养改善了hiPSC染色体的稳定性和多能性.
  • 这种优化的培养环境降低了氧化酸化和衰老路径的调节.
  • 物理氧培养诱导了线粒体复合体组成的显著重新连接,提高了hiPSC适合研究的适度.