通过从基因组编辑中获得的复合人体脂肪素加强信号通路的激活
Eunhui Yoo1, Hee Jung Choi1, Jae Yong Han1
1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Journal of biotechnology
|September 28, 2024
概括
蛋白衍生的人类脂蛋白 (hADPN) 有效地激活关键信号通路,包括ERK1/2,Akt和p38 MAPK. 在基因组编辑的中,这种新的生产方法为产生功能性hADPN提供了优越的替代方案.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 阿迪波涅丁 (ADPN) 通过ERK1/2,Akt和p38 MAPK等信号通路调节细胞和代谢功能.
- 在常规系统中,产生功能性重组人体脂肪蛋白 (hADPN) 是一个挑战.
- 现有的ADPN激动酶在稳定性,溶解性和受体亲和性方面存在局限性.
研究的目的:
- 为了评估卵白 (EW) 衍生hADPN的信号通路激活与商业hADPN,激素和球状ADPN相比.
- 评估基因组编辑生物反应器系统对hADPN生产的有效性.
主要方法:
- 用EW衍生的hADPN,商业hADPN,ADPN激活和球状ADPN对待HEK293细胞.
- 通过评估蛋白质酸化来测量ERK1/2,Akt和p38 MAPK通路的激活.
- 分析了EW衍生的hADPN成分 (六合体,高分子量多元体).
主要成果:
- 在15分钟内,EW衍生的hADPN显著化ERK1/2在血清饥饿的HEK293细胞中.
- 来自EW和商业hADPN都诱导了比和球状ADPN更大的通路激活 (ERK1/2,Akt,p38 MAPK).
- 来自EW的hADPN显示了研究信号通路的最高激活.
结论:
- 源自EW的hADPN强烈激活ERK1/2,Akt和p38的MAPK信号级联.
- 基因组编辑的提供了一个有效和有利的系统来产生功能性的hADPN.
- 该系统为当前的重组蛋白质生产方法提供了一个有希望的替代方案.
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