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海湾 Argopecten irradians 的染色体级基因组组合

Denis Grouzdev¹, Emmanuelle Pales Espinosa¹, Stephen Tettelbach²

¹School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, 11794-5000, USA.

Scientific data

|September 28, 2024

概括

我们向您介绍了海湾贝 (Argopecten irradians) 的第一个染色体级基因组组,这是水产养殖和生态系统的关键物种. 这种高质量的参考基因组将有助于理解它的进化和改进育种策略.

科学领域:

海洋生物学海洋生物学
基因组学就是基因组学.
水产养殖科学水产养殖科学

背景情况:

湾 (Argopecten irradians) 在生态和商业上具有重要意义,在中国已经建立了水产养殖业,在美国有本土种群.
一个高质量的参考基因组对于理解贝生物和促进遗传改进至关重要.

研究的目的:

为了生成和注释海湾 (Argopecten irradians) 的染色体级参考基因组组.
为研究进化动力学,适应机制和基因组辅助育种提供基础资源.

主要方法:

用PacBio和Hi-C测序数据进行基因组组.
使用Hi-C分析进行支架和染色体识别.
基因预测和注释,包括重复元素分析和BUSCO完整性评估.

主要成果:

845.9 Mb的基因组组合包括1503个支架,其中92.9%的数据在16个最大的支架中对应于染色体.
鉴定了33772个编码蛋白质的基因,并分析了重复元素 (36.2%).
高基因组完整性 (96.2%的BUSCO得分) 和包括完整的线粒体基因组.

结论:

染色体水平的基因组组合代表了海湾研究的重大进步.
该资源将支持保护工作,水产养殖发展以及对环境变化适应的研究.

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Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.