相关实验视频

Updated: Jun 11, 2025

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

核酸结合亲和力和N-m-Tolyl-4-Chlorophenoxyacetohydroxamicacid的抗氧化活性

Rubi Khilari¹, Sohilkhan Chauhan², Mamta Tripathi³

¹School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur-492010, Chhattisgarh, Raipur, India.

Scientific reports

|September 28, 2024

概括

这项研究表明,N-m-tolyl-4-chlorophenoxyaceto胺酸插入DNA和RNA,取代乙基化物. 该化合物还表现出抗氧化活性,并防止DNA损伤.

科学领域:

生物化学生物化学
分子生物学分子生物学
药用化学医学化学

背景情况:

胺酸是有机酸的一类,具有多种天然和合成来源.
了解它们与核酸的相互作用对于开发新的治疗剂至关重要.

研究的目的:

研究N-m-tolyl-4-chlorophenoxyaceto酸与小牛胸腺DNA (ct-DNA) 和酵母RNA (t-RNA) 的结合机制.
评估该化合物的抗氧化特性及其防止DNA损伤的能力.

主要方法:

使用紫外可见光谱,光辐射分析和粘度测量来研究结合相互作用.
使用乙基化物 (EB) 进行了竞争性结合试验.
使用AutoDock4软件进行计算模拟.
使用DPPH激素清理和β-胡卜素氨酸试验来评估抗氧化活性.
凝电泳用于评估DNA损伤预防.

主要成果:

光谱和粘度测量数据表明,胺酸与ct-DNA和t-RNA有间接的结合模式.
该化合物有效地将EB从核酸中取代并灭光.
计算了强结合常量 (K_b) 和负结合的自由能量值.
该化合物表现出显著的抗氧化活性,并保护DNA免受损伤.

结论:

在N-m-tolyl-4-chlorophenoxyaceto中,胺酸通过间隔作用与ct-DNA和t-RNA结合.
该化合物具有有前途的抗氧化和DNA保护性质,需要进一步研究治疗应用.

关键词:

有约束力的约束力在Ct-DNA/t-RNA中.胺酸是一种胺酸.互动互动互动

更多相关视频

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Published on: May 13, 2020

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

Published on: June 10, 2022

相关概念视频

ortho–para-Directing Activators: –CH3, –OH, –&NoBreak;NH2, –OCH3

ortho–para-Directing Activators: –CH₃, –OH, –⁠NH₂, –OCH₃

All ortho–para directors, excluding halogens, are activating groups. These groups donate electrons to the ring, making the ring carbons electron-rich. Consequently, the reactivity of the aromatic ring towards electrophilic substitution increases. For instance, the nitration of anisole is about 10,000 times faster than the nitration of benzene. The electron-donating effect of the methoxy group in anisole activates the ortho and para positions on the ring and stabilizes the corresponding...