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识别功能性circRNAs调节卵巢卵泡发育在山羊

Jie Liu^1,2,3, Guanghang Feng^1,2,3, Conghui Guo^1,2,3

¹Herbivore Laboratory, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.

|September 28, 2024

概括

循环RNAs (circRNAs) 调节了山羊的毛囊发育. 发现了一种新型的circRNA,circCFAP20DC,通过激活视网膜母细胞瘤 (RB) 途径来增强颗粒细胞的增殖.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
分子内分泌学分子内分泌学

背景情况:

循环RNAs (circRNAs) 是哺乳动物生物过程中的关键调节者.
羊卵泡发育中circRNAs的作用在很大程度上仍然没有被描述.
了解circRNA功能是改善山羊生殖特征的关键.

研究的目的:

研究羊毛囊发育中的circRNAs的表达和功能.
在莱州山羊中识别小毛囊和大毛囊之间的差异表达的circRNAs.
阐明皮囊粒状细胞增殖中circRNAs的调节机制.

主要方法:

小 (1-3毫米) 和大 (>6毫米) 羊毛囊的RNA测序.
生物信息分析包括基因本体学 (GO) 和KEGG通路丰富.
竞争性内源性RNA (ceRNA) 网络的构建.
实验验证使用EDU测定,流细胞计,qPCR和西式涂抹.

主要成果:

鉴定了152个差异表达的circRNA,其中101个在大毛囊中上调调节.
丰富的途径包括卵巢类固醇生成,GnRH信号传递和自.
一种新型的circRNA,circCFAP20DC,被发现并被证明可以增强颗粒状细胞的增殖.
circCFAP20DC激活了视网母细胞瘤 (RB) 途径,促进细胞周期进展 (G1到S阶段).

结论:

不同的circRNAs在山羊的毛囊发育中起着重要的作用.
新型circRNAcircCFAP20DC通过RB通路促进了颗粒细胞的增殖.
这些发现为管理山羊毛囊生长的分子机制提供了洞察力.

关键词:

毛囊的发育毛囊的发展莱州山羊 - 莱州山羊有关RNA测序的RNA测序生殖生殖的繁殖电路CFAP20DCDC电路是一个电路.

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