在混合培养发酵过程中,微波辅助有机酸和绿色的生产
Maximilian Barth1, Magdalena Werner1, Pascal Otto1
1Institute for Waste Management and Circular Economy, TUD Dresden University of Technology, Pirna, Germany.
Biotechnology for biofuels and bioproducts
|September 28, 2024
概括
在无氧消化中对种子污泥施加的热冲击增强了绿色和短链脂肪酸的生产. 微波辅助热冲击为工业应用提供了可重复和可预测的替代炉处理方法.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 环境科学 环境科学
- 微生物生态学 微生物生态学
背景情况:
- 将无氧消化纳入生物基础工业可以提高可持续性.
- 两级消化器可以生产有价值的工业产品,如绿色和短链脂肪酸.
- 热冲击显示了提高产品产量的潜力,但实际应用需要进一步研究.
研究的目的:
- 调查热冲击处理在无氧消化过程中的实际适用性.
- 为了比较微波辅助的热冲击与传统的基于烤箱的治疗方法.
- 评估热冲击对微生物群落结构和产品形成的影响.
主要方法:
- 批量实验是在37°C的5L反应堆中进行的.
- 微波和烤箱热冲击 (80°C,5-10分钟) 应用于种子污泥.
- 分析了气体生产,化学参数和微生物分类概况 (16S rRNA基因测序).
主要成果:
- 最高的生产率 (1.09mol H2/mol hexose) 是通过在50g/L基底负荷下进行热冲击来实现的.
- 微波辅助的热冲击显示了与烤箱处理相比的可比生产率,并提高了可重复性 (29%的变化).
- 热冲击预处理显著地将微生物群落转移到Clostridia,并促进了黄油酸的形成 (高达24.01 g/L).
结论:
- 整个种子污泥的热冲击处理适用于工业无氧消化.
- 微波辅助热冲击是一种有希望的,可重复的替代工业实施的烤箱处理方法.
- 这一过程在经济上是可行的,使用高温热 (COP 4.3).
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