在循环 (Phe-Pro) 生产中对C4-二碳酸盐的监管机制
Xinyan Xu1, Liu Liu1, Lihui Xu2
1State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.
在Burkholderia seminalis中,循环Phe-Pro (cFP) 的产生受到C4-二碳酸盐的负面调节. 这些化合物增强非核糖体合成酶 (NRPS) 基因的fur介导抑制,减少cFP生物合成.
科学领域:
- 微生物学 微生物学
- 生物化学 生化学
- 植物病理学 植物病理学
背景情况:
- 循环Phe-Pro (cFP) 是一种具有显著抗真菌,抗菌和抗病毒特性的循环二,显示出对植物疾病的生物控制的潜力.
- cFP是由非核糖体合成酶 (NRPS) 生产的,但其生物合成调节尚未得到充分理解.
- Burkholderia seminalis菌株R456产生cFP,是研究其调节的目标.
研究的目的:
- 阐明管理Burkholderia seminalis中CFP生物合成的监管机制.
- 确定影响cFP生产的遗传因素及其对植物病原体的对抗作用,如Fusarium graminearum (Fg).
主要方法:
- 对Burkholderia seminalis R456的997个Tn5突变体进行查,以识别对Fg.seminalis R456增强对抗性的菌株.
- 高性能液体染色学 (HPLC) 和液体染色学-质谱学 (LC-MS) 用于cFP的识别和量化.
- 对Dct运输系统和NRPS集群基因的基因淘汰和补充研究.
- 酵母一杂交试验,研究毛皮蛋白与NRPS基因促进体的结合.
主要成果:
- 八种Tn5突变对Fg表现出增强的对抗性;突变88产生可检测的cFP,抑制Fg.
- 干扰Dct基因 (参与C4-二碳酸盐运输) 导致cFP产量增加.
- 外源C4-二碳酸盐降低了野生类型R456.6中cFP的产量.
- 敲除NRPS基因2061显著降低了cFP的生产.
- 毛皮蛋白与2061基因的促进体结合,这种结合由C4-二碳酸盐增强.
- 毛皮基因淘汰导致2061基因的上调,并增加了cFP的产量.
结论:
- C4-二碳酸盐在Burkholderia seminalis中负面调节了cFP的合成.
- 这种调节通过NRPS基因2061的增强Fur介导抑制发生.
- 了解这种机制可以帮助优化用于农业应用的CFP生产.
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